Hi-Affi™ In Vitro Cell based Chemokine Receptor Functional Characterization Service

Chemokine Receptors Overview

Chemokine receptors are serpentine G-protein-coupled receptors that are expressed by both cancer and stromal cells. The receptors and their ligands are important inflammatory mediators, controlling leukocyte extravasation and directing migration into inflamed and diseased organs. Targeting overexpressed chemokine receptors directly kills tumor cells and has a favorable effect on hematological malignancies, making them appealing tractable targets for the pharmaceutical sector.

Fig.1 Chemokine receptors in cancer therapy.^1,3

Our Capability to Support Global Customers

As a leader in GPCRs functional assay, Creative Biolabs provides a top-ranking Hi-Affi™ in vitro cell-based chemokine receptor functional assay service to advance drug development in cancer therapy. Our Hi-Affi™ in vitro cell-based chemokine receptor functional assay service includes fluorescence and multiple second messenger readouts, including cAMP and calcium flux, and allows us to assay or analyze compounds' ability to modulate chemokine receptor activation and subsequent redistribution inside cells. We can assist worldwide clients in the following ways:

Identify the localization and activation of chemokine receptors
Design experiments
Choose the best analytical methodology
Develop and validate the specific assay(s)

Fig.2 The workflow of our Hi-Affi™ in vitro cell-based chemokine receptor functional assay. (Creative Biolabs Original

Why Choose Us

All-encompassing to diverse categories of chemokine receptors.
Our Hi-Affi™ in vitro cell-based chemokine receptor functional assay combines with cell-based assays and an easy-to-operate process, which boosts drug development with no risk and is suitable for all categories of chemokine receptors.
A broad spectrum of cell types serves as useful tools.
Prominent in the field of cell research, we provide a wide range of cell lines including CHO cell lines, HEK293T cell lines, etc. And we took advantage of these cell lines to accomplish numerous projects.
Rapid time frames advance our assay service.
We employ within-the-firm kits to ensure high-quality, on-time delivery of global customers' projects and present the desirable results in 1-2 weeks.
Experienced professional scientists guarantee quality first.
Each of our customers may be certain that their projects will go forward smoothly thanks to our cutting-edge research team.
Integrated bespoke services
We provide a full-around customized Hi-Affi™ in vitro cell-based chemokine receptor functional assay service from sample processing to data visualization with the most up-to-date technical solutions.

Case Show

Case: This study investigated biased agonism at human CC chemokine receptors CCR4, CCR7, and CCR10, examining how different chemokine ligands preferentially activate G protein- and adaptor protein-mediated signaling pathways within the same cellular context, given the promiscuous nature of chemokine receptor interactions and the known biased signaling of these receptors. The results demonstrate the considerable variations between the intracellular signals evoked.

Adaptor protein recruitment assay

Fig.4 Chemokine ligands differentially stimulate adaptor protein recruitment to CCR4, CCR7, and CCR10. Fig.2 The recruitment of adaptor protein to the chemokine receptors CCR4, CCR7, and CCR10 stimulated by different chemokine ligands.^2,3

G protein activation assay

Fig.5 Chemokine ligands differentially stimulate G protein activation via CCR4, CCR7, and CCR10. Fig.3 The G protein activation via CCR4, CCR7, and CCR10 stimulated by different chemokine ligands.^2,3

cAMP synthesis assay

Fig.6 Chemokine ligands differentially inhibit cAMP synthesis via CCR4, CCR7, and CCR10. Fig.4 The cAMP synthesis inhibition via CCR4, CCR7, and CCR10 stimulated by different chemokine ligands.^2,3

Work with Creative Biolabs

Fig.3 Contact flowchart. (Creative Biolabs Original)

In the field of GPCR functional identification, Creative Biolabs is constantly optimizing and innovating and is committed to presenting the most ideal results for each customer. If you are interested in our Hi-Affi™ in vitro cell-based chemokine receptor functional assay service, please feel free to contact us. We are sincerely looking forward to working with you.

References

Mollica Poeta, Valeria, et al. "Chemokines and chemokine receptors: new targets for cancer immunotherapy." Frontiers in Immunology 10 (2019): 379.
Lim, Herman D., et al. "Systematic assessment of chemokine signaling at chemokine receptors CCR4, CCR7 and CCR10." International Journal of Molecular Sciences 22.8 (2021): 4232.
Distributed under Open Access License CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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