FluxControl™ ABCB1 (P-gp) ELISA Kit

CAT#: ITS-0326-YL42
Product Type: Assay Kit
Species: Human, Rat, Mouse
Brand: FluxControl™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies ABCB1 protein using a sandwich ELISA method.
Brand
FluxControl™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
This high-sensitivity sandwich ELISA kit facilitates the precise detection of ABCB1 protein within cell membrane preparations. By enabling accurate quantification, the assay supports critical research into drug efflux transport kinetics and the identification of potential drug-drug interactions. It serves as a vital tool for investigators aiming to understand how this transporter influences the systemic disposition and overall pharmacokinetic profiles of many diverse pharmaceutical compounds and substrates.
Features
Optimized sandwich ELISA designed for high-sensitivity detection of ABCB1, providing essential data for analyzing efflux transporter kinetics and assessing drug-drug interaction risks.
Process Relevance
Measuring P-glycoprotein levels to standardize membrane preparations for accurate efflux transport kinetic and interaction assays.
Application Stage
Drug discovery, transport studies, and safety assessment.
Applications
Detect P-glycoprotein levels in cell membranes to analyze drug efflux transport kinetics and identify potential drug-drug interactions.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Sample Type
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Research Areas
ADME; Drug Metabolism; Pharmacokinetics
Size
96 tests
Data Output Type
Absorbance (OD)
LOD
Rat: 0.168 ng/mL
Human: 0.076 ng/mL
Mouse: 0.078 ng/mL
Species
Human, Rat, Mouse
Storage
Upon receipt, please follow the storage recommendations in the USER MANUAL to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
This membrane-bound efflux transporter actively pumps a wide range of substrates out of cells, significantly limiting drug absorption and distribution. Its function is a key determinant of the pharmacokinetic profile of numerous clinical therapeutics.
For Research Use Only | Not For Clinical Use
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