MetaVital™ Rat Flavin Containing Monooxygenase 1 (FMO1) ELISA Kit

CAT#: ITS-0326-WB14
Product Type: ELISA Kit
Species: Rat
Target: Rat flavin-containing monooxygenase 1 (FMO1)
Brand: MetaVital™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures rat FMO1 protein levels in biological samples using a sensitive sandwich ELISA assay.
Brand
MetaVital™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
MetaVital™ Rat Flavin Containing Monooxygenase 1 (FMO1) ELISA Kit enables precise quantification of FMO1 protein in rat tissues, plasma, or cell extracts. Employing a highly specific sandwich ELISA format with optimized antibody pairs, the assay facilitates sensitive detection of this Phase I metabolic enzyme, supporting pharmacokinetic studies, toxicology research, and investigations of xenobiotic biotransformation pathways.
Features
High-affinity antibody pairs ensure selective detection of rat FMO1 with minimal cross-reactivity to other flavin-containing monooxygenase isoforms.
Sensitive microplate-based sandwich ELISA enables accurate measurement of low-abundance FMO1 protein across diverse tissue, plasma, and cellular samples.
Pre-optimized reagents and standardized protocol ensure reproducible results, reducing variability across experiments and sample types.
Assay design allows convenient integration into high-throughput pharmacological, toxicological, and metabolic research workflows.
Process Relevance
Quantifies FMO1 expression to evaluate Phase I metabolic activity and xenobiotic detoxification in rat models.
Application Stage
Pharmacokinetic profiling, drug metabolism research, toxicology studies, and metabolic enzyme characterization in preclinical models.
Applications
Quantitative analysis of FMO1 protein in rat tissues, plasma, or cultured cells for metabolic enzyme profiling, drug metabolism studies, ADME profiling studies, and xenobiotic detoxification research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Rat flavin-containing monooxygenase 1 (FMO1)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Drug metabolism & pharmacokinetics (ADME), toxicology, pharmacology, enzymology, preclinical biomarker research, xenobiotic metabolism, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using colorimetric sandwich ELISA detection.
Assay Range
0.156 ng/ml - 10 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Flavin-containing monooxygenase 1 (FMO1) is a cytosolic Phase I enzyme responsible for N-, S-, and P-oxidation of xenobiotics and endogenous compounds. It contributes to metabolic clearance and detoxification in rodent models, providing critical insights into drug metabolism, preclinical pharmacology, and chemical safety evaluation.
Pathways
FMO1 catalyzes Phase I oxidative metabolism of xenobiotics, converting lipophilic substrates into more polar metabolites. These reactions often complement cytochrome P450-mediated oxidation. Metabolites generated by FMO1 are subsequently processed by Phase II conjugation enzymes, facilitating detoxification, excretion, and modulation of pharmacological activity.
For Research Use Only | Not For Clinical Use
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