CytoFinal™ Cow Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit

CAT#: ITS-0326-WB76
Product Type: ELISA Kit
Species: Cow
Target: Bovine alanine aminotransferase 1 (ALT1/GPT)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
High-sensitivity ELISA kit for quantifying ALT1 (GPT) protein levels in cow biological samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Cow Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit enables precise detection of ALT1 in bovine serum, plasma, tissues, and cell lysates. Utilizing a robust sandwich ELISA format with high-affinity antibodies, this kit ensures accurate quantification of ALT protein for veterinary diagnostics, metabolic research, and preclinical liver function studies
Features
Species-specific antibodies for bovine ALT1 provide high specificity and minimal cross-reactivity with related aminotransferase isoforms.
Sensitive sandwich ELISA detection allows quantification of low-abundance ALT protein in complex bovine samples.
96-well microplate format facilitates high-throughput analysis compatible with standard ELISA readers.
Pre-optimized reagents and calibrated standards ensure reproducible, reliable results across multiple experiments.
Process Relevance
Measures ALT1 levels in bovine samples to monitor liver health, hepatocellular stress, and metabolic enzyme dynamics.
Application Stage
Veterinary research, livestock metabolic studies, liver toxicity assessment, and preclinical biomarker evaluation.
Applications
Quantifies ALT1 protein in bovine serum, plasma, tissues, and cultured cells for liver function assessment, metabolic pathway analysis, ADME profiling studies, and veterinary toxicology studies.
Target
Bovine alanine aminotransferase 1 (ALT1/GPT)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Veterinary hepatology, livestock metabolism, veterinary toxicology, pharmacokinetics (ADME), preclinical metabolism research, animal health biomarker development, translational veterinary medicine, etc.
Size
96 tests
Data Output Type
Quantitative ALT1 protein concentration determined via absorbance in a sandwich ELISA assay.
Assay Range
1.56 ng/ml - 100 ng/ml
LOD
< 0.63 ng/ml
Species
Cow
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
ALT1 catalyzes the transfer of an amino group from alanine to α-ketoglutarate, generating pyruvate and glutamate. In cows, ALT levels serve as sensitive indicators of liver function and metabolic status, providing essential insights for veterinary and preclinical research.
Pathways
ALT1 participates in the alanine cycle, linking amino acid metabolism to hepatic gluconeogenesis. Produced pyruvate integrates into the TCA cycle or gluconeogenic pathways, connecting nitrogen metabolism with cellular energy production in bovine physiology.
For Research Use Only | Not For Clinical Use
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