CytoFinal™ Human Lactate Dehydrogenase (LDH) ELISA Kit

CAT#: ITS-0326-WB106
Product Type: ELISA Kit
Species: Human
Target: Human Lactate Dehydrogenase (LDH) antigen
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures human Lactate Dehydrogenase (LDH) levels in serum, plasma, and tissue samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Human Lactate Dehydrogenase (LDH) ELISA Kit offers a sensitive, specific in vitro assay to quantify LDH concentrations in serum, plasma, tissue homogenates, and other biological fluids, providing a reliable tool for metabolic and cytotoxicity studies.
Features
High sensitivity detection with a lower limit of <0.06 ng/mL, enabling precise quantification of low-abundance LDH molecules.
Excellent specificity with minimal cross-reactivity, ensuring accurate measurement of LDH without interference from analogues.
Sandwich ELISA format with pre-coated 96-well plates, TMB colorimetric readout, and HRP-conjugated detection reagents for robust quantitative analysis.
Broad test range of 0.156-10 ng/mL suitable for diverse sample types, including serum, plasma, cell lysates, and culture supernatants.
Process Relevance
Enables precise monitoring of LDH levels to assess metabolic status and cytotoxicity in experimental workflows.
Application Stage
Applicable in drug discovery, toxicology screening, and in-process clinical research.
Applications
Suitable for quantitative ELISA detection of LDH in serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants for ADME profiling studies.
Target
Human Lactate Dehydrogenase (LDH) antigen
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Cellular metabolism, toxicology, oncology, clinical biomarker studies, pharmacokinetics (ADME), metabolic disorder research, etc.
Size
96 tests
Data Output Type
Quantitative measurement of enzyme concentration using colorimetric readout (endpoint assay).
Assay Range
0.156 ng/ml - 10 ng/ml
LOD
< 0.06 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase (LDH) is a key cytoplasmic enzyme catalyzing the reversible conversion of pyruvate to lactate, linking glycolysis and cellular respiration. LDH levels reflect tissue metabolic activity, hypoxia, and cell damage, serving as a critical biomarker in clinical and research settings.
Pathways
LDH participates in anaerobic glycolysis, connecting pyruvate metabolism to lactate production. Its activity is influenced by NAD+/NADH balance, upstream glycolytic flux, and downstream lactate utilization in gluconeogenesis or mitochondrial respiration.
For Research Use Only | Not For Clinical Use
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