CytoFinal™ Pig Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB97
Product Type: ELISA Kit
Species: Pig
Target: Aspartate Aminotransferase, Cytoplasmic / AST (GOT1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures cytoplasmic AST (GOT1) levels in pig biological samples using a sensitive ELISA assay.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Pig Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit enables accurate in vitro quantification of GOT1 in serum, plasma, tissue homogenates, cell lysates, and culture supernatants. Designed for reliable detection in diverse biological matrices, it supports research in liver injury, metabolic studies, and cancer biology.
Features
High specificity and sensitivity ELISA format accurately detects cytoplasmic AST (GOT1) across multiple pig-derived sample types.
Compatible with serum, plasma, tissue, and cell lysates, ensuring flexible experimental design and broad applicability.
Includes optimized reagents and controls, providing consistent, reproducible results and minimizing inter-assay variability.
Long shelf-life and stability under recommended conditions, enabling convenient storage and reliable assay performance over 12 months.
Process Relevance
Monitors GOT1 enzyme levels to evaluate liver function, metabolic activity, and tumor-associated metabolic adaptations.
Application Stage
Preclinical research, translational studies, and in vitro biomarker assessment.
Applications
Supports quantitative ELISA-based measurement of GOT1 in pig serum, plasma, tissues, and cultured cells for hepatology, oncology, and ADME profiling studies.
Target
Aspartate Aminotransferase, Cytoplasmic / AST (GOT1)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Enzymology, hepatology, tumor immunology, pharmacokinetics (ADME), infection immunity, cancer metabolism, etc.
Size
96 tests
Data Output Type
Quantitative enzyme concentration measurement (endpoint ELISA format).
Assay Range
31.2 pg/ml - 2000 pg/ml
LOD
< 12.6 pg/ml
Species
Pig
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
GOT1 catalyzes the reversible conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate, central to amino acid metabolism. It plays critical roles in neurotransmission, liver function, and cancer cell energy metabolism, making it a key biomarker for liver injury and tumor metabolic profiling.
Pathways
GOT1 participates in amino acid and nitrogen metabolism, linking the TCA cycle and glutamate production. Dysregulation affects oxaloacetate availability, influencing pyruvate synthesis and cellular bioenergetics, particularly in hepatic fibrosis and tumor metabolism.
For Research Use Only | Not For Clinical Use
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