CytoFinal™ High Sensitivity Mouse Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit

CAT#: ITS-0326-WB73
Product Type: ELISA Kit
Species: Mouse
Target: Mouse alanine aminotransferase 1 (ALT1/GPT)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Highly sensitive ELISA kit for precise quantification of mouse ALT1 (GPT) protein in biological samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ High Sensitivity Mouse Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit provides a robust immunoassay for accurate measurement of ALT1 protein in mouse serum, plasma, tissue lysates, and cultured cells. Utilizing a high-affinity sandwich ELISA format, the kit enables detection of low-level ALT expression, supporting preclinical studies in liver injury, metabolism, and toxicology research.
Features
Ultra-sensitive sandwich ELISA format allows reliable quantification of low-abundance ALT protein in complex mouse biological matrices.
Mouse-specific high-affinity antibody pair ensures precise detection of ALT1 with minimal cross-reactivity to related aminotransferase isoforms.
96-well microplate assay design enables high-throughput analysis compatible with standard ELISA plate readers for efficient workflow.
Optimized reagents and calibrated standards provide reproducible signal development and consistent quantitative performance across experiments.
Process Relevance
Measures ALT protein to monitor hepatocellular damage and metabolic enzyme regulation in mouse preclinical models.
Application Stage
Preclinical research, toxicology assessment, metabolic disease modeling, and biomarker validation studies.
Applications
Quantitative detection of ALT protein in mouse serum, plasma, tissues, and cultured cells for liver injury studies, toxicology evaluation, metabolic pathway analysis, ADME profiling studies, and preclinical biomarker research.
Target
Mouse alanine aminotransferase 1 (ALT1/GPT)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, toxicology, metabolic disease research, pharmacology, pharmacokinetics (ADME), preclinical biomedical research, molecular metabolism, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement based on absorbance signals generated in a sandwich ELISA format.
Assay Range
0.156 ng/ml - 10 ng/ml
LOD
< 0.06 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alanine aminotransferase 1 (ALT1) catalyzes the reversible transamination between alanine and α-ketoglutarate, producing pyruvate and glutamate. As a hepatocyte-enriched enzyme, circulating ALT levels serve as biomarkers of liver injury and metabolic disturbances in mouse models.
Pathways
ALT1 functions in the alanine cycle, linking peripheral amino acid metabolism with hepatic gluconeogenesis. Pyruvate produced enters the TCA cycle or gluconeogenic pathways, integrating nitrogen metabolism with cellular energy production.
For Research Use Only | Not For Clinical Use
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