45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-381-2994
Europe: 44-207-097-1828
Fax: 1-631-207-8356

Electric Transfection

Creative Biolabs is a remarkable servicer in the CAR production. Based on the advanced technology and powerful research & development team, we have exploited one-stop CAR development platform to meet your needs. We promise to provide every client with our intimate service and ideal results.

Relying on a simple process, electroporation emerges as a powerful tool for the genetic modification of diverse cell types based on the transient disruption of cell membrane via exposure to an electric field, which allows charged molecules to enter the cell. It can be used with most cell types, especially T cells, yielding a high frequency of both stable transformation and transient gene expression. Electroporation is easier to operate compared with the methods of viral transfection and transposons transfection. The main advantage of electric transfection is its applicability for transient and stable transfection of T cells. Moreover, electric transfection is easy and rapid to transfect a large number of T cells in a short time.

On the other hand, the main potential disadvantage of electroporation is the excessive cell death and the low transfection efficiency, which is varied with cell types and electroporation conditions (voltage, wave profile, time of the pulse, and buffer composition). Therefore, Creative Biolabs has optimized the process of electric transfection by distributing the electrical pulse equally among the cells and maintaining a stable pH and modifying the pulse and field strength parameters to reduce the loss as described above.

Our clients will benefit from:

Efficient transfection of T cells
Transfecting by electric process without any harmful viability
Transfecting a large number of T cells in a short time
Saving time and cost

The Process of Electric Transfection

Fig.1 The Process of Electric Transfection

Creative Biolabs has the capability to enable you to free up your time for core work and project. Our service can be designed to meet your special needs if you have any requirements. If you are interested in our service, please contact us by E-mail and our team will get back to you as soon as possible.

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994
Fax: 1-631-207-8356

Tel: 44-207-097-1828


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