Transposons Transfection Services

The field of genetic engineering is continuously evolving, moving beyond the limitations of early viral and non-viral delivery systems. While CRISPR-Cas technologies have revolutionized targeted gene editing, the challenge of achieving stable, long-term, and high-efficiency genomic integration of large transgenes remains a significant hurdle in both basic research and therapeutic development. Viral vectors, though efficient, are often constrained by cargo size, immunogenicity, and complex production logistics. Non-viral methods like lipofection or electroporation frequently result in transient expression, limiting their utility for applications requiring sustained genetic modification. At Creative Biolabs, with over two decades of pioneering work in molecular and cell biology, we have perfected the art and science of transposon-based gene delivery.

Fig.1 Schematic design of the transposon systems (PB, SB, and Tol2) application in bovine somatic and germ cell^1,2

Our suite of Transposons Transfection Services provides a robust, versatile, and scalable platform to overcome these challenges, enabling seamless genomic integration for your most ambitious cellular engineering projects.

Our Comprehensive Service Portfolio

We offer an end-to-end solution, tailoring our services to meet the specific needs of each client's project, from initial vector design to final clone validation.

Why Partner with Creative Biolabs? The Definitive Advantages

Choosing our services provides you with a strategic edge, grounded in scientific excellence and operational reliability.

Unparalleled Expertise: Our team of PhD-level scientists brings over 20 years of direct experience in transposon biology, vectorology, and cell line development. We navigate complex project challenges with proven strategies.

Superior Integration Efficiency: Our proprietary formulations of hyperactive transposase systems (e.g., SB100X, piggyBac variants) ensure exceptionally high integration rates, leading to a greater yield of stable clones.

Precision and Footprint-Free Engineering: A key differentiator of our piggyBac-based services is the ability to perform "footprint-free" excision, allowing for clean removal of the transgene, which is invaluable for iPSC reprogramming and reversible genetic studies.

Robust and Scalable Process: From a small-scale research project to GMP-compatible manufacturing for pre-clinical studies, our processes are designed for scalability and reproducibility.

Integrated, End-to-End Support: We are your single point of contact for the entire project lifecycle, from conceptual design to the delivery of validated, banked cell lines, saving you time and streamlining internal resources.

Our Streamlined Project Workflow

We believe in transparency and clear communication throughout our collaboration.

Diverse Applications Across Biotechnology

Our Transposon Transfection Services are a foundational technology with broad applicability.

	Stable Cell Line Development For high-yield recombinant protein production (e.g., antibodies, vaccines) and long-term functional studies
	Gene and Cell Therapy Research Engineering of CAR-T cells, stem cells, and other therapeutic cell types with stable transgene expression for pre-clinical development
	Functional Genomics & Screens Creating genome-wide lentiviral-transposon hybrid libraries for high-throughput gain-of-function or loss-of-function genetic screens.
	Induced Pluripotent Stem Cell (iPSC) Engineering Safe and efficient integration of reprogramming factors or corrective genes into patient-derived iPSCs.
	Disease Modeling Generating isogenic cell lines with specific disease-associated mutations to study pathogenesis and identify novel therapeutics.

Client Testimonials: Evidence of Excellence

• "Creative Biolabs's piggyBac service was instrumental in generating our master cell line for a complex bispecific antibody. Their team's expertise in handling large DNA constructs and their rigorous clone screening process saved us months of internal work. The resulting cell line shows exceptional stability and productivity." – Director of Cell Line Development, Biopharma
• "We engaged Creative Biolabs to engineer patient-derived iPSCs for a rare disease model. Their ability to achieve high-efficiency, footprint-free integration of our reporter construct was exactly what we needed. The scientific collaboration and support throughout the project were outstanding." – Principal Investigator, Academic Research Institute
• "We struggled for months with low efficiency in primary T-cells. Creative Biolabs's optimized transfection protocol delivered the high-efficiency, stable CAR integration we needed for our pre-clinical models, with exceptional cell viability." – Project Lead, Cell Therapy Biotech
• "Their team's deep expertise in piggyBac technology was the differentiator. The precise, footprint-free excision in our iPSC line was flawless, enabling a critical phase of our regenerative medicine program." – Research Fellow, Academic Medical Center
• "Outstanding service from start to finish. The comprehensive molecular data provided with the final clonal cell line gave us complete confidence in the model for our high-throughput screening campaign." – Head of Oncology Discovery

Addressing Your Scientific Inquiries

References

1. Kwon, Dong-Hyeok et al. "Application of transposon systems in the transgenesis of bovine somatic and germ cells." BMC veterinary research vol. 18,1 156. 27 Apr. 2022, https://doi.org/10.1186/s12917-022-03252-1
2. Distributed under Open Access license CC BY 4.0, without modification.