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Transposons Transfection

Based on the outstanding expertise and rich experience, Creative Biolabs has developed transposons transfection system which is different from virial transfection. Academic researchers in biotechnology can carry out the whole procession of CAR producing. With manufacturing and quality control of high standards, Creative Biolabs provides custom transfection service for our clients.

Transposons are a non-virial alternative for stable gene transfer. There are two main vectors, namely Sleeping Beauty (SB) and PiggyBac (PB), using as transposons in the producing of CARs. As a tool of genetic engineering, transposons have made great contributions to vertebrate genetic studies from gene delivery to gene discovery. Transposition requires transposon DNA and the transposase enzyme. It’s the movement of the DNA fragment of transposon in a way called “cut and paste”. The mechanism of SB and PB has four steps: 1) the transposase enzyme recognizes and binds to the IR in transposon; 2) A synaptic complex is formed by the pairing and binding of the repeat elements at both ends of the transposon under the interaction of the transposase subunit.; 3) the shear of the donor occurs under the action of the transposon; 4) the target position of the transposable element is reintegrated. The transposase of SB and PB is composed of the DNA binding domain, the transposable catalytic domain and unclear positioning signal (NLS). In the process of the transposon, transposable element and the special factor encoding by the host code are required. These factors become the transposable molecular node to control the transposon process.

The SB system is a safer alternative to viral vectors due to its non-pathogenic origin, inherently low enhancer activity, and minimal epigenetic modifications at the insertion site. By contrast, PB system shows a large cargo capacity and more efficient transposition activity in preclinical studies. Significantly, the safety of PB system derives CAR-T cells over those from the SB system.

The Process of Transposon Transfection

Fig.1 The Process of Transposon Transfection

The service of transposon transfection optimized by Creative Biolabs has the benefits of higher capacity of carrying target DNA. Further, efficiency of gene movement and higher safety than the virial vectors make CARs production more reliable. Therefore, this service will be powerful and useful in the CAR production.

Table.1 The Core Benefits of SB and PB

Sleeping Beauty (SB) PiggyBac (PB)
Easier to insert into the donor More efficient
Higher activity Will not leave the extra sequence
High capacity, high efficiency and high safety

Creative Biolabs has the capability to enable you to free up your time for core work and project. Our service can be designed to meet your special needs if you have any requirements. If you are interested in our service to support development of CARs, please contact us and learn more by sending E-mail and our team will get back to you as soon as possible.

Reference

Richard A. et al. Genetic Modification of T Cells. Biomedicines 2016, 4, 9.

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CONTACT US

USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-871-5806
Fax: 1-631-207-8356
Email:

Europe
Ringstrasse 4, 64401 Gross-Bieberau, Germany
Tel: 44-207-097-1828

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