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MUC1

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Product List Background Anti-MUC1 CAR-T Services Anti-MUC1 CAR-T In Vitro Assay ServicesAnti-MUC1 CAR-T In Vivo Assay Services

Product List

Creative Biolabs provides various Anti-MUC1-related products to support global customers’ projects, please feel free to contact us for further communication.

Background

MUC1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. It is overexpressed in a variety of epithelial cancers and plays a crucial role in the progression of the disease. Tumor-associated MUC1 differs from the MUC1 expressed in normal cells with regard to its biochemical features, cellular distribution, and function. Based on these features of MUC1 between normal and tumor cells, MUC1 has been investigated as one of the hot targets for antibody and engineering CAR T cells for cancer therapy.

Schematic representation of the recognition of different domains by different antibodies. Fig.1 Schematic representation of the recognition of different domains by different antibodies.1

Associated Disease

Lung cancer

Pancreas cancer

Breast cancer

MUC1-expressing tumors

Viral infection

Others

Anti-MUC1 CAR-T Services

Anti-MUC1 CAR-T In Vitro Assays

Anti-MUC1 CAR-T Expression Test

For CART research, Creative Biolabs is dedicated to providing several innovative CAR detection reagents for CAR expression validation. Meanwhile, we also provide a comprehensive range of assay services to support your CART projects. For example, validation of anti-MUC1 CAR expression by flow cytometry is usually a classical assay format in MUC1 CAR therapy projects, providing a lot of intuitive information to clients.

Anti-MUC1 CAR expression evaluation in transduced human T cells by FACS analysis of Myc-tag expression. Anti-MUC1 CAR cells were gated by CD4 or CD8 channel, and then detected Myc-tag expression.. Fig.2 Anti-MUC1 CAR expression evaluation in transduced human T cells by FACS analysis of Myc-tag expression. Anti-MUC1 CAR cells were gated by CD4 or CD8 channel, and then detected Myc-tag expression.2

Anti-MUC1 CAR-T Cytokine Release Test

The cytokine release test is a valuable assay in CART validation and characterization, which provides valuable insights into the activated and lytic phenotype of CAR T cells. Common types of cytokine tests include IFN-γ, TNF-α, IL-2, Granzyme B, along with many other cytokines. Creative Biolabs specializes in providing comprehensive cytokine release test services for global clients by many approaches, including ELISA, CBA, and FACS staining intracellularly.

IFN-γ and Granzyme B secretion detection of anti-MUC1 CAR-T cells co-cultured with tMUC1high HCC70 cells by FACS staining intracellularly for IFN-γ and Granzyme B. (Zhou, et al., 2019). Fig.3 IFN-γ and Granzyme B secretion detection of anti-MUC1 CAR-T cells co-cultured with tMUC1high HCC70 cells by FACS staining intracellularly for IFN-γ and Granzyme B.2

Cytokines panel detection of anti-MUC1 CAR-T cells co-cultured with tMUC1high HCC70 cells at E:T ratio of 2:1 by human cytokine array. (Zhou, et al., 2019) Fig.4 Cytokines panel detection of anti-MUC1 CAR-T cells co-cultured with tMUC1high HCC70 cells at E:T ratio of 2:1 by human cytokine array.2

Anti-MUC1 CAR-T In Vitro Cytotoxicity Assay

CAR T cell-mediated tumor-killing test is an important indicator for CART research. Creative Biolabs offers in vitro cytotoxicity assay services to support the evaluation of anti-MUC1 CAR-T cell therapy by various approaches, such as radioactive chromium release method, bioluminescence method, and flow cytometry method. In the testing service, we will optimize the experimental conditions according to customers' special needs to get more desirable data results.

In vitro cytotoxicity activity of anti-MUC1 CAR-T cells against TNBC tumor cells. (Zhou, et al., 2019) Fig.5 In vitro cytotoxicity activity of anti-MUC1 CAR-T cells against TNBC tumor cells. (A) tMUC1 expression validation of several TNBC cell lines by flow cytometry. (B) In vitro cytotoxicity validation of MUC1 CAR T cells against TNBC tumor cell at E:T ratio of 5:1 for 3 days' co-culture.2

Anti-MUC1 CAR-T In Vivo Assays

Efficacy Test of Anti-MUC1 CAR-T

For in vivo tumor killing analysis, Creative Biolabs provides many optimized mouse models and various detection solutions to speed clients' CART research. At the same time, our in vivo services also cover a variety of phenotypic analyses on cells.

In vivo anti-tumor validation of MUC1 CAR-T cells against HCC70 tumor cells. (Zhou, et al., 2019) Fig.6 In vivo anti-tumor validation of MUC1 CAR-T cells against HCC70 tumor cells. (A) Anti-MUC1 CAR-T cells inhibit HCC70 tumor growth in NSG mice by a single injection in vivo. (B) Tumor weight analysis of resected tumor mass on day 57 at endpoint.2

Toxicity Evaluation Anti-MUC1 CAR-T

In addition, Creative Biolabs also provides comprehensive solutions for the toxicity assessment of CAR-T cells to ensure the safety and efficacy of the CART cells, such as on-target toxicity assessment, and off-target toxicity assessment.

For more MU1 CAR-T related assays, please don't hesitate to contact us directly.

References

  1. Bose, Mukulika, and Pinku Mukherjee. "Potential of anti-MUC1 antibodies as a targeted therapy for gastrointestinal cancers." Vaccines 8.4 (2020): 659.
  2. Zhou, Ru.; et al. "CAR T cells targeting the tumor MUC1 glycoprotein reduce triple-negative breast cancer growth." Frontiers in immunology 10 (2019): 1149.

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CAT Product Name Target Species Antibody Clone Antibody Host Receptor Construction Vector Type Targeting Cell Type CAR Vector Type Inquiry & Datasheet
XS-0123-ZP82 Anti-MUC1 (SN-101) h(scFv-CD3ε) TRuC, pCDTRC1 Human SN-101 Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP83 Anti-MUC1 (2D9) h(scFv-CD3ε) TRuC, pCDTRC1 Human 2D9 Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP84 Anti-MUC1 (014E) h(scFv-CD3ε) TRuC, pCDTRC1 Human 014E Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP85 Anti-MUC1 (2B11) h(scFv-CD3ε) TRuC, pCDTRC1 Human 2B11 Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP86 Anti-MUC1 (2H11) h(scFv-CD3ε) TRuC, pCDTRC1 Human 2H11 Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP87 Anti-MUC1 (DMB5F3) h(scFv-CD3ε) TRuC, pCDTRC1 Human DMB5F3 Humanized scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP88 Anti-MUC1 (H23) h(scFv-CD3ε) TRuC, pCDTRC1 Human H23 Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP89 Anti-MUC1 (BOS7D10) h(scFv-CD3ε) TRuC, pCDTRC1 Human BOS7D10 Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP90 Anti-MUC1 (SM3) h(scFv-CD3ε) TRuC, pCDTRC1 Mouse SM3 Human scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP91 Anti-MUC1 (TAB-004) h(scFv-CD3ε) TRuC, pCDTRC1 Human TAB-004 Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP92 Anti-MUC1 (C595) h(scFv-CD3ε) TRuC, pCDTRC1 Human C595 Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP93 Anti-MUC1 (Epitumomab) h(scFv-CD3ε) TRuC, pCDTRC1 Human Epitumomab Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP94 Anti-MUC1 (HMFG2) h(scFv-CD3ε) TRuC, pCDTRC1 Human HMFG2 Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP95 Anti-MUC1 (Nacolomab) h(scFv-CD3ε) TRuC, pCDTRC1 Human Nacolomab Mouse scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0123-ZP460 Anti-MUC1 (5-24) h(scFv-CD3ε) TRuC, pCDTRC1 Mouse 5-24 Camel scFv-CD3ε Lentiviral vector T cell   Add to Cart   Datasheet
XS-0323-LX144 Anti-MUC1 VHH(X3XH144) h(CD28-41BB-CD3ζ) CAR, pCDCAR1 Vector Human X3XH144 VHH-CD28-41BB-CD3ζ Lentiviral vector T cell CAR-T   Add to Cart   Datasheet
XS-0323-LX145 Anti-MUC1 VHH(X3XH145) h(CD28-41BB-CD3ζ) CAR, pCDCAR1 Vector Human X3XH145 VHH-CD28-41BB-CD3ζ Lentiviral vector T cell CAR-T   Add to Cart   Datasheet
XS-0323-LX146 Anti-MUC1 VHH(X3XH146) h(CD28-41BB-CD3ζ) CAR, pCDCAR1 Vector Human X3XH146 VHH-CD28-41BB-CD3ζ Lentiviral vector T cell CAR-T   Add to Cart   Datasheet
XS-0323-LX147 Anti-MUC1 VHH(X3XH147) h(CD28-41BB-CD3ζ) CAR, pCDCAR1 Vector Human X3XH147 VHH-CD28-41BB-CD3ζ Lentiviral vector T cell CAR-T   Add to Cart   Datasheet
XS-0323-LX513 Anti-MUC1 VHH(X3XH144) h(ICOS-41BB-CD3ζ) CAR, pCDCAR1 Vector Human X3XH144 VHH-ICOS-4-1BB-CD3ζ Lentiviral vector T cell CAR-T   Add to Cart   Datasheet
XS-0823-LX79 Anti-hMUC1 (M3) ICD(CD28-OX40-CD3ζ) CAR-MA, pAd5f35 Vector Human M3 Adenoviral vectors   Add to Cart   Datasheet
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