Sleeping Beauty Transposon System for CAR-T

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Creative Biolabs has been a world-famous scientific partner in the field of antibody discovery and cell-based immunotherapy for years. Now, our scientists are proud to offer advanced Sleeping Beauty (SB) transposon system as an important non-virial transfection system for CAR-T gene delivery.

Background

Viral vectors play important roles in traditional CAR-T cell therapy nowadays. Although they are promising, imitations still exist. Firstly, using viral vector carries a risk of insertional oncogenesis or clonal dominance. Meanwhile, when administered directly in vivo, viral vectors may cause unwanted immunogenicity. Moreover, it is cumbersome and costly for the manufacturing of viral vector since living cells are required and the quality control is complicated as well as the downstream processing. Therefore, we bring out the SB transposon system as an alternative of viral vectors to circumvent some of these limitations.

Introduction of SB Transposon System

SB transposon system is a wonderful synthetic DNA transposon which is composed of two components, a transposase (360 amino acids) and a transposon. It is designed to precisely integrate defined DNA sequences into a TA dinucleotide of vertebrate animals. It is a simple, cut-and-paste manner to translocate DNA from one site to another. As shown in Fig 1, plasmid 1 is loaded with a transgene (the CAR gene), named transposon, surrounded by inverted repeats (IRs) that contain short direct repeats (DRs). Plasmid 2 encodes the enzyme (transposase) which can recognize the sequences from plasmid 1 and cut the transposon out of the plasmid 1. Then the CAR gene will be successfully delivered into the targeted cell (T cell) cytoplasm and inserted randomly into TA dinucleotide base pairs of the recipient DNA sequence. By this means, stable integration and reliable long-term expression of the CAR genes in T cells is possible and attractive.

Schema of the transposon/transposase SB system.

Fig 1. Schema of the transposon/transposase SB system. (Heiblig M, et.al. 2015)

Highlight Features of Our System and Our Service

This system is composed solely of DNA resulting in cheaper costs of production and delivery compared with viral vectors.
Large cargo capacity up to 12 kb.
Minimal immunogenicity in vivo and well incorporated with our CAR platforms.
Higher transposition activity in mammalian cells compared with some other transposon systems.
Ph.D. level scientists work closely with each other to guarantee the highest success rate of every project.
Firm quality control of every step to ensure reliable quality

Empowered by high-end technologies and platforms, Creative Biolabs has served numerous customers from research institution and pharmacology industry. We are more than happy to share our experience, platforms, and resources with you to promote each project closer to clinical trials. Please don't hesitate to contact us for more details and our team will get back to you as soon as possible.

Reference

Heiblig M, et.al. (2015). Adoptive immunotherapy for acute leukemia: New insights in chimeric antigen receptors. World J Stem Cells. 7(7), 1022-38.