PiggyBac Transposon System for CAR-T

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Based on years of experience in the field of CAR-T cell therapy and antibody discovery, Creative Biolabs has established several all-round platforms to help researchers from all over the world to develop effective products. With non-stop exploration, we present the state-of-the-art piggyBac (PB) transposon system as an important non-virial transfection system for CAR-T gene delivery.

Background

Viral vectors have been widely applied in traditional CAR-T cell therapy to treat genetic cancers. However, the maximum cargo size of viral vectors is restricted, thus hampering the transfer of larger designed CAR gene. Besides, safety and immunogenicity are also the major worries of the viral vectors. In addition, using living cells for the production of virus complicates the quality control and downstream processing. Fully understand new needs, Creative Biolabs has built up the non-virial PB transposon system to overcome these restrictions.

Introduction of PB Transposon System

There are two classes of transposons according to different mechanism of transposition, retrotransposons and DNA transposons. Among them, DNA transposons have become a research hotspot since they can transpose via a "cut-and-paste" mechanism, without creating a second copy of the DNA during transposition. PB transposon system belongs to DNA transposons. It is composed of a transposase (594 amino acids) and a transposon-like other transposon systems. The PB transposon is a movable genetic element that efficiently transposes between vectors and chromosomes. When performing transposition function, the PB transposase will recognize the transposon-specific inverted terminal repeats (ITRs) sequences which are located on both ends of the transposon vector. After that, the PB transposase moves the contents from its original positions and integrates them into TTAA chromosomal sites efficiently. In CAR gene delivery, the designed CAR genes are incorporated in the transposon vector. Of note, PB has the highest transposition activity in mammalian cells as well as the Sleeping Beauty (SB) transposon system.

Schema of the transposon/transposase PB system.

Fig 1. Schema of the transposon/transposase PB system. (Zhao S, et.al. 2016)

Highlight Features of Our System and Service

The PB transposon-based system exhibited a good safety profile, which makes it suitable for clinical translation.
Large cargo capacity up to 100 kb.
The transposition is efficient, safe and stable.
Lower costs of production compared with viral vectors.
Well trained technicians and expert scientists ensure the highest success rate of every project.
Tight quality control of every step to offer high-quality products

Creative Biolabs has been a world-famous service provider over the past years. We are eager to share our technologies, platforms, and scientific teams with you. With the help of us, there is a larger possibility to push your project closer to clinical trials. Please don't hesitate to contact us for more details and our team will get back to you as soon as possible.

Reference

Zhao S, et.al. (2016). PiggyBac transposon vectors: the tools of the human gene encoding. Transl Lung Cancer Res. 5(1), 120-5.