ConjuLink™ Universal Sulfotransferase Activity Kit

CAT#: ITS-0326-WB9
Product Type: Assay Kit
Species: General (All species)
Brand: ConjuLink™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Measures sulfotransferase enzymatic activity using a universal assay compatible with diverse substrates and biological samples.
Brand
ConjuLink™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
ConjuLink™ Universal Sulfotransferase Activity Kit provides a sensitive platform for quantifying sulfotransferase (SULT) activity across multiple biological systems. Utilizing optimized reaction chemistry and a universal detection strategy, the assay enables measurement of sulfate transfer from activated donor molecules to diverse substrates. This kit supports enzymatic characterization, metabolic profiling, and drug metabolism studies involving sulfation pathways.
Features
Universal detection chemistry enables monitoring of sulfotransferase activity across multiple enzyme isoforms and substrate classes with high analytical sensitivity.
Compatible with purified enzymes, microsomes, and cell or tissue lysates, supporting flexible experimental designs in metabolic enzyme studies.
Microplate-based assay format allows scalable throughput for compound screening and enzyme characterization in drug discovery programs.
Optimized reagent composition ensures stable cofactor utilization and reproducible sulfation measurements across diverse experimental conditions.
Process Relevance
Measures sulfotransferase-mediated conjugation to evaluate metabolic stability and compound detoxification potential.
Application Stage
Drug discovery, ADME profiling, enzymology research, and metabolic pathway characterization.
Applications
Quantification of sulfotransferase activity in purified enzymes or biological samples, screening of enzyme inhibitors or modulators, metabolic pathway analysis, and ADME studies evaluating sulfation-mediated metabolism.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Sample Type
Tissue homogenates, cell lysates, and other biological fluids.
Assay Measures
Quantitative
Research Areas
Drug metabolism & pharmacokinetics (ADME), glycobiology, enzymology, toxicology, pharmacology, xenobiotic metabolism, etc.
Data Output Type
Quantitative enzymatic activity measurement based on sulfation-dependent signal generation in microplate assays.
Species
General (All species)
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Sulfotransferases (SULTs) are Phase II metabolic enzymes that catalyze the transfer of sulfate groups to small molecules, hormones, and xenobiotics. This modification increases molecular polarity and promotes elimination from the body. Because sulfation regulates the activity and clearance of many endogenous metabolites and pharmaceuticals, accurate measurement of sulfotransferase activity is critical for metabolic and pharmacological research.
Pathways
Sulfotransferases function in the Phase II xenobiotic metabolism pathway, transferring sulfate from the universal donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to target molecules. This conjugation step often follows oxidative Phase I metabolism mediated by cytochrome P450 enzymes. Together, these sequential metabolic processes control detoxification, metabolic clearance, and regulation of bioactive compounds.
For Research Use Only | Not For Clinical Use
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