CytoFinal™ Chicken Lactate Dehydrogenase A (LDHA) ELISA Kit

CAT#: ITS-0326-WB119
Product Type: ELISA Kit
Species: Chicken
Target: Chicken Lactate Dehydrogenase A (LDHA)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitative sandwich ELISA kit for sensitive detection of chicken LDHA in biological samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Chicken Lactate Dehydrogenase A (LDHA) ELISA Kit is a highly sensitive sandwich ELISA designed for the quantitative measurement of chicken LDHA in serum, plasma, tissue homogenates, and cell culture supernatants. Utilizing a pre-coated antibody microplate and HRP-based colorimetric detection, this assay enables reliable measurement of LDHA levels to support metabolic, physiological, and disease-related studies.
Features
High-sensitivity sandwich ELISA enabling reliable quantification of chicken LDHA across a dynamic range of 0.78-50 ng/mL.
Optimized antibody pair provides excellent target specificity with minimal cross-reactivity, ensuring accurate detection in complex biological matrices.
Ready-to-use 96-well microplate format with pre-coated capture antibodies supports streamlined workflow and improved assay reproducibility.
Compatible with diverse sample types including serum, plasma, tissue homogenates, and cell culture supernatants for versatile experimental applications.
Process Relevance
Enables precise monitoring of LDHA levels to evaluate metabolic shifts, tissue damage, and cellular glycolytic activity.
Application Stage
Basic research, metabolic studies, disease biomarker exploration, and experimental validation.
Applications
Quantitative detection of LDHA in serum, plasma, tissue homogenates, and culture supernatants for metabolic studies, enzyme biomarker research, cellular metabolism analysis, ADME profiling studies, and avian physiological investigations.
Target
Chicken Lactate Dehydrogenase A (LDHA)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Metabolic regulation, glycolysis research, avian physiology, cellular metabolism, veterinary biomedical research, tissue injury biomarkers, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of LDHA concentration based on optical density at 450 nm.
Assay Range
0.78 ng/ml - 50 ng/ml
LOD
< 0.28 ng/ml
Species
Chicken
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase A (LDHA) is a cytosolic enzyme that catalyzes the conversion of pyruvate to lactate during anaerobic glycolysis. Its expression reflects cellular metabolic state and energy demand. Altered LDHA levels are associated with metabolic stress, tissue injury, and changes in cellular bioenergetics.
Pathways
LDHA plays a central role in the glycolysis and lactate fermentation pathway, converting pyruvate to lactate while regenerating NAD+ required for continuous glycolytic flux. It functions downstream of glucose metabolism and influences cellular energy balance, hypoxia responses, and metabolic adaptation.
For Research Use Only | Not For Clinical Use
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