P450-Metric™ Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2) ELISA Kit

CAT#: ITS-0326-YL27
Product Type: Assay Kit
Species: Human
Brand: P450-Metric™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies CYP21A2 using a double-antibody sandwich ELISA.
Brand
P450-Metric™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
This advanced double-antibody sandwich ELISA kit is meticulously engineered for the reliable quantitative analysis of Cytochrome P450 21A2 expression within adrenal tissue lysates and homogenates. Highlighting remarkable analytical sensitivity, this research-use-only assay empowers researchers to comprehensively investigate localized steroidogenic drug metabolism. It is an essential laboratory asset for determining potential off-target endocrine disruptions and analyzing the biotransformation of steroid-based pharmaceuticals in preclinical ADME and toxicology.
Features
Utilizing a highly sensitive double-antibody sandwich design, this ELISA accurately quantifies CYP21A2, supporting comprehensive preclinical research on localized steroidogenic drug metabolism and xenobiotic interactions.
Process Relevance
Measuring CYP21A2 to monitor steroidal agent progression and evaluate endocrine safety margins during preclinical testing.
Application Stage
Preclinical drug discovery, ADME profiling, and pharmacokinetic research
Applications
Quantify specific target enzyme levels in cell lysates, tissue homogenates, and biological fluids using this robust immunoassay.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Sample Type
Serum, plasma, tissue homogenates, cell lysates
Research Areas
ADME; Endocrine Pharmacology; Toxicology; Drug Metabolism
Size
48 / 96 tests
Data Output Type
Absorbance (OD)
LOD
Human: 0.055 ng/mL
Species
Human
Storage
Upon receipt, please follow the storage recommendations in the USER MANUAL to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Operating as a specialized steroid 21-hydroxylase within the adrenal cortex, Cytochrome P450 21A2 is indispensable for the biosynthesis of major steroid hormones. In ADME and toxicology, its monitoring is vital to analyze the metabolic fate of steroid-based pharmaceuticals and detect off-target endocrine disruption.
For Research Use Only | Not For Clinical Use
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