CypMaster™ CYP3A4 Fluorometric Activity Assay Kit

CAT#: ITS-0326-WB5
Product Type: Assay Kit
Species: Human
Target: Cytochrome P450 3A4 (CYP3A4)
Brand: CypMaster™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures CYP3A4 enzymatic activity in microsomes, tissues, and cells using a sensitive fluorometric detection method.
Brand
CypMaster™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CypMaster™ CYP3A4 Fluorometric Activity Assay Kit provides a rapid and reliable method for quantifying cytochrome P450 3A4 enzymatic activity in biological samples. The assay employs a fluorogenic substrate converted by CYP3A4 into a highly fluorescent product, enabling precise detection using a standard microplate reader. This system supports efficient evaluation of enzyme activity, inhibitor screening, and drug metabolism studies in ADME research.
Features
Fluorogenic substrate generates a strong fluorescent signal upon CYP3A4-mediated metabolism, enabling highly sensitive enzymatic activity quantification.
Compatible with liver microsomes, cell lysates, and tissue extracts, supporting flexible experimental designs across diverse biological systems.
Parallel inhibitor-controlled reactions enable accurate determination of CYP3A4-specific activity by eliminating non-specific metabolic background signals.
Optimized for microplate-based workflows, enabling scalable throughput for drug metabolism studies and compound screening applications.
Process Relevance
Measures CYP3A4 metabolic activity to evaluate drug metabolism potential and predict metabolic liabilities during candidate development.
Application Stage
Drug discovery, ADME profiling, metabolism studies, and drug-drug interaction risk assessment.
Applications
Quantification of CYP3A4 enzymatic activity in liver microsomes, cultured cells, and tissue lysates; inhibitor or inducer screening; drug metabolism evaluation; pharmacokinetic and ADME profiling studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Cytochrome P450 3A4 (CYP3A4)
Detection Method
Fluorescent
Sample Type
Tissue lysate, microsomes, cell lysate
Assay Measures
Quantitative
Research Areas
Drug metabolism & pharmacokinetics (ADME), toxicology, pharmacology, drug discovery, hepatology, xenobiotic metabolism, enzymology, etc.
Size
200 tests
Data Output Type
Quantitative fluorescence-based enzyme activity measurement using kinetic or endpoint microplate detection.
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Cytochrome P450 3A4 (CYP3A4) is one of the most abundant drug-metabolizing enzymes in the human liver and intestine. It catalyzes oxidative biotransformation of numerous xenobiotics and pharmaceuticals, playing a central role in Phase I metabolism. Because CYP3A4 metabolizes a large proportion of clinically used drugs, its activity significantly influences pharmacokinetics and drug safety.
Pathways
CYP3A4 participates in the cytochrome P450-mediated xenobiotic metabolism pathway, catalyzing oxidative reactions that convert lipophilic compounds into more hydrophilic metabolites for excretion. This process relies on NADPH-dependent electron transfer and interacts with other metabolic enzymes, including CYP3A5 and CYP2C isoforms, influencing drug clearance, metabolic activation, and drug-drug interaction potential.
For Research Use Only | Not For Clinical Use
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