P450-Metric™ Cytochrome P450 27A1 (CYP27A1) ELISA Kit

CAT#: ITS-0326-YL12
Product Type: Assay Kit
Species: Human
Brand: P450-Metric™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies CYP27A1 using a double-antibody sandwich ELISA.
Brand
P450-Metric™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
This optimized double-antibody sandwich ELISA kit is precisely tailored for the reliable quantitative determination of Cytochrome P450 27A1 in experimental biological samples. Offering rigorous analytical specificity and high reproducibility, this research-use-only assay empowers pharmacologists to investigate complex mitochondrial sterol metabolism. It stands as an essential laboratory tool for assessing the pharmacokinetic disposition of lipid-lowering therapeutics and xenobiotic-induced metabolic alterations in comprehensive ADME research.
Features
Utilizing a robust sandwich ELISA format, this assay offers precise CYP27A1 quantification, facilitating advanced research into hepatic sterol metabolism and the pharmacokinetics of lipid-modulating therapeutics.
Process Relevance
Assessing CYP27A1 expression to map mitochondrial clearance pathways for steroidal drugs and vitamin D analogs.
Application Stage
Preclinical drug discovery, ADME profiling, and pharmacokinetic research
Applications
Perform reliable quantitative analysis of target expression in cell lysates and relevant biological matrices using this immunoassay.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Sample Type
Serum, plasma, tissue homogenates, cell lysates
Research Areas
ADME; Drug Metabolism; Lipid Metabolism; Pharmacokinetics
Size
48 / 96 tests
Data Output Type
Absorbance (OD)
LOD
Human: 0.055 ng/mL
Species
Human
Storage
Upon receipt, please follow the storage recommendations in the USER MANUAL to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Cytochrome P450 27A1 is a multifunctional mitochondrial enzyme critical to the alternative bile acid synthesis pathway and the metabolism of sterol compounds. Its monitoring is necessary to understand the pharmacokinetic disposition of steroidal drugs and xenobiotic-induced alterations in cholesterol clearance.
For Research Use Only | Not For Clinical Use
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