CytoFinal™ Rat Lactate Dehydrogenase B (LDHB) ELISA Kit

CAT#: ITS-0326-WB114
Product Type: ELISA Kit
Species: Rat
Target: Rat Lactate Dehydrogenase B (LDHB)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitative ELISA kit for measuring rat Lactate Dehydrogenase B (LDHB) in biological samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Rat Lactate Dehydrogenase B (LDHB) ELISA Kit enables sensitive and specific quantification of LDHB protein in rat-derived biological samples. Using a high-performance sandwich ELISA format with HRP-based colorimetric detection, this kit allows reliable measurement of LDHB levels in serum, plasma, tissue lysates, and cell culture extracts for metabolic and disease-related research.
Features
High-specificity sandwich ELISA utilizing validated antibody pairs enables selective detection of rat LDHB with minimal cross-reactivity toward other LDH isoforms.
Optimized assay sensitivity and dynamic linear range allow reliable quantification of LDHB across multiple biological matrices and experimental conditions.
HRP-TMB colorimetric detection generates stable signals compatible with standard microplate readers for straightforward and efficient laboratory data acquisition.
Comprehensive kit configuration including precoated microplates, standards, wash buffers, and detection reagents ensures convenient operation and reproducible experimental results.
Process Relevance
Measures LDHB levels to investigate metabolic balance and tissue-specific energy metabolism in experimental models.
Application Stage
Preclinical disease research, metabolic pathway analysis, biomarker discovery, and pharmacological studies.
Applications
Detection of rat LDHB in serum, plasma, tissue lysates, and cell extracts for metabolic pathway studies, cardiac research, cytotoxicity analysis, ADME profiling studies, and biomarker investigations.
Target
Rat Lactate Dehydrogenase B (LDHB)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Cardiovascular research, metabolic biology, toxicology, cellular metabolism, disease biomarker discovery, pharmacokinetics (ADME), pharmacological studies, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of LDHB protein concentration based on ELISA absorbance.
Assay Range
0.312 ng/ml - 20 ng/ml
LOD
< 0.13 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase B (LDHB) is one of the major isoforms of the LDH enzyme family and is highly expressed in oxidative tissues such as heart and brain. Unlike LDHA, LDHB preferentially catalyzes the conversion of lactate to pyruvate, supporting aerobic metabolism. Its expression level is often associated with tissue metabolic state and disease conditions.
Pathways
LDHB participates in the glycolysis-gluconeogenesis metabolic network by catalyzing the conversion of lactate to pyruvate while regenerating NADH. This reaction links lactate metabolism to mitochondrial oxidative phosphorylation. LDHB activity therefore contributes to cellular energy homeostasis and metabolic adaptation in highly oxidative tissues.
For Research Use Only | Not For Clinical Use
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