CytoFinal™ Low Sample Volume Human Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB95
Product Type: ELISA Kit
Species: Human
Target: Cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies cytoplasmic AST (GOT1) in human samples with minimal volume for sensitive metabolic and liver studies.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Low Sample Volume Human AST (GOT1) ELISA Kit enables accurate detection of cytoplasmic AST in small-volume human serum, plasma, tissues, or cell lysates. This kit supports clinical research, hepatocellular assessment, and metabolic monitoring with high sensitivity and reproducibility, even in limited sample volumes.
Features
Requires minimal human sample volume while providing precise and reproducible cytoplasmic AST quantification.
High specificity discriminates cytoplasmic AST from mitochondrial isoforms, reducing cross-reactivity and measurement error.
Optimized reagents and buffers deliver consistent results across multiple human sample types.
Rapid, colorimetric format allows high-throughput analysis while maintaining sensitive detection for low-abundance AST.
Process Relevance
Enables sensitive cytoplasmic AST monitoring in small human samples, improving hepatocellular assessment and metabolic profiling.
Application Stage
Suitable for clinical research, preclinical studies, biomarker analysis, and toxicology evaluations.
Applications
Precise cytoplasmic AST quantification in human serum, plasma, tissues, or cell lysates for liver function evaluation and ADME profiling studies.
Target
Cytoplasmic Aspartate Aminotransferase (AST/GOT1)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, clinical diagnostics, translational research, metabolism, pharmacokinetics (ADME), enzymology, biomarker discovery, toxicology, pharmacology, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of cytoplasmic AST levels in low-volume human samples.
Assay Range
15.6 ng/ml - 1000 ng/ml
LOD
< 5.5 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
AST/GOT1 catalyzes the reversible transamination of aspartate and α-ketoglutarate, linking amino acid metabolism with energy production. Cytoplasmic AST levels provide insights into hepatocellular integrity, metabolic flux, and clinical safety assessments.
Pathways
AST functions in the malate-aspartate shuttle, regulating cytoplasmic-mitochondrial redox balance. It integrates with TCA cycle activity and amino acid homeostasis, influencing energy metabolism in hepatocytes and other metabolically active tissues.
For Research Use Only | Not For Clinical Use
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