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MemDX™ Human IL2RG & IL2RB & IL2RA BaF3 Cell Line with STAT5-Luc Reporter (CAT#: S01YF-1222-KX882)

Datasheet
This product is a reporter cell line. It stably expresses Human IL2RG & IL2RB & IL2RA to provide cell models for research and screening applications.

Product Information

  • Target Protein
  • IL2RG & IL2RB & IL2RA
  • Target Protein Species
  • Human
  • Accession Number
  • NM_000206.3; NM_000878.5; NM_000417.3
  • Protein Tag
  • Tag-free
  • Host Cell Type
  • BaF3
  • Target Classification
  • Immune Checkpoint
  • Target Family
  • Immune Checkpoint
  • Target Research Area
  • Autoimmune Research; Immunology Research
  • Related Diseases
  • Severe Combined Immunodeficiency

Product Properties

  • Morphology
  • Suspension
  • Assay Types
  • Drug screening and biological assays
  • Assay Reporter
  • STAT5-Luc
  • Resistance
  • Puromycin; Hygromycin
  • Stability
  • 10 passages
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 5x10⁶ cells
  • Form
  • Frozen cells
  • Freeze Medium
  • 70% RPMI 1640 + 20% FBS + 10% DMSO
  • Culture Medium
  • RPMI 1640 + 10% FBS + 1mg/mL Hygromycin + 1µg/mL Puromycin
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • Interleukin 2 receptor subunit gamma
  • Introduction
  • The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.
  • Alternative Names
  • IL2RG; P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1; cytokine receptor common subunit gamma; CD132 antigen; IL-2 receptor subunit gamma; IL-2R subunit gamma; common cytokine receptor gamma chain; gammaC; interleukin 2 receptor, gamma; nonfunctional common cytokine receptor gamma chain; Interleukin 2 receptor subunit gamma

Case Studies

Customer reviews and Q&As    

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