CytoFinal™ Low Sample Volume Human Lactate Dehydrogenase (LDH) ELISA Kit

CAT#: ITS-0326-WB109
Product Type: ELISA Kit
Species: Human
Target: Human lactate dehydrogenase
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitative ELISA kit for measuring human lactate dehydrogenase using minimal sample volumes.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Low Sample Volume Human Lactate Dehydrogenase (LDH) ELISA Kit is designed for sensitive quantification of human LDH in biological samples while requiring minimal sample input. Using a high-performance sandwich ELISA format with HRP-mediated colorimetric detection, this kit enables reliable measurement of LDH levels in serum, plasma, cell culture supernatants, and tissue extracts, supporting cytotoxicity and metabolic research.
Features
Highly sensitive sandwich ELISA design enables accurate detection of human LDH while requiring minimal biological sample volumes.
Optimized antibody pairing ensures strong specificity and minimal cross-reactivity, enabling reliable quantification across complex biological matrices.
Colorimetric HRP-TMB detection provides robust signal generation compatible with standard microplate readers for straightforward laboratory workflows.
Complete assay kit including precoated plates, calibrated standards, and optimized buffers supports reproducible results and convenient implementation.
Process Relevance
Measures LDH release to monitor cellular damage, cytotoxicity, and metabolic alterations during experimental workflows.
Application Stage
Drug discovery, biomarker research, disease modeling, and preclinical translational studies.
Applications
Quantification of human LDH in serum, plasma, cell culture supernatants, and tissue lysates for cytotoxicity evaluation, metabolic studies, disease biomarker research, ADME profiling studies, and therapeutic response analysis.
Target
Human lactate dehydrogenase
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Oncology metabolism, pharmacokinetics (ADME), clinical biomarker discovery, immunology and inflammation, toxicology and drug safety, cardiovascular research, cellular metabolism, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of LDH concentration determined by ELISA absorbance values.
Assay Range
3.12 ng/ml - 200 ng/ml
LOD
< 1.14 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase (LDH) is a ubiquitous cytoplasmic enzyme involved in cellular energy metabolism. It catalyzes the reversible conversion of pyruvate to lactate while regenerating NAD+ during glycolysis. Because LDH is rapidly released upon membrane disruption, extracellular LDH levels are widely used as biomarkers of tissue injury, cytotoxicity, and metabolic stress.
Pathways
LDH functions at a critical step of glycolysis, interconverting pyruvate and lactate while maintaining NAD+ availability for continued glycolytic flux. This process becomes particularly important under hypoxic conditions. Elevated LDH activity is associated with metabolic reprogramming pathways, including aerobic glycolysis observed in proliferating cells and tumor microenvironments.
For Research Use Only | Not For Clinical Use
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