CytoFinal™ High Sensitivity Rat L-Lactate Dehydrogenase A Chain (LDHA) ELISA Kit

CAT#: ITS-0326-WB122
Product Type: ELISA Kit
Species: Rat
Target: Rat lactate dehydrogenase A chain
Brand: CytoFinal
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitative ELISA kit for measuring rat LDHA protein levels in biological samples.
Brand
CytoFinal
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ High Sensitivity Rat L-Lactate Dehydrogenase A Chain (LDHA) ELISA Kit is a sensitive sandwich ELISA designed for quantitative detection of LDHA in rat biological samples. This kit enables accurate measurement of LDHA expression levels in serum, plasma, tissue lysates, or cell culture supernatants, supporting studies of metabolic regulation, cellular damage, and disease-related metabolic alterations.
Features
High-sensitivity sandwich ELISA enables reliable detection of rat LDHA across a broad dynamic concentration range.
Optimized antibody pair ensures strong specificity toward rat LDHA with minimal cross-reactivity.
Ready-to-use reagents and standardized protocol enable rapid and reproducible quantification.
Compatible with multiple rat sample types including serum, plasma, cell lysates, and tissue extracts.
Process Relevance
Measures LDHA levels to evaluate metabolic activity, glycolytic flux, and cellular damage in experimental systems.
Application Stage
Applicable in disease research, metabolic studies, biomarker discovery, and experimental pharmacology.
Applications
Quantitative detection of rat LDHA in serum, plasma, tissue lysates, or cultured cells for metabolic profiling, disease biomarker evaluation, cytotoxicity studies, ADME profiling studies, and glycolysis-related pathway research.
Target
Rat lactate dehydrogenase A chain
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Cellular metabolism, glycolysis regulation, cancer metabolism, toxicology, pharmacokinetics (ADME), inflammation biology, metabolic biomarker discovery, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement via absorbance-based ELISA endpoint detection.
Assay Range
0.156 ng/ml - 10 ng/ml
LOD
< 0.069 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ⅘ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase A (LDHA) is a critical enzyme in anaerobic glycolysis that catalyzes the conversion of pyruvate to lactate while regenerating NAD+. It plays an essential role in maintaining cellular energy production under hypoxic conditions. Altered LDHA expression is commonly associated with metabolic reprogramming, tissue injury, and disease progression.
Pathways
LDHA functions in the glycolysis and lactate fermentation pathways, linking cytosolic energy metabolism with cellular redox balance. It acts downstream of glucose metabolism and supports NAD+ regeneration for sustained glycolytic flux. LDHA activity is frequently regulated by hypoxia-responsive signaling pathways such as HIF-1α-mediated metabolic adaptation.
For Research Use Only | Not For Clinical Use
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