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Magic™ Human FLAG tagged GLP1R HEK293T Cell Line, Calcium flux assay (CAT#: S01YF-1022-KX359)

Datasheet
This product is a HEK293T stable cell line. It stably expresses FLAG tagged GLP1R to provide cell models for research and screening applications.

Product Information

  • Target Protein
  • GLP1R
  • Target Protein Species
  • Human
  • Accession Number
  • NM_002062
  • Protein Tag
  • FLAG-tag at N-terminus
  • Host Cell Type
  • HEK293T
  • Target Classification
  • GPCR
  • Target Family
  • Glucagon
  • Target Research Area
  • Cancer Research; Diabetes Research; Digestive and Renal Research
  • Related Diseases
  • Insulinoma; Hyperglycemia

Product Properties

  • Assay Types
  • Calcium flux assay
  • Resistance
  • Puromycin
  • Stability
  • Stable for a minimum of 2 months in continuous culture
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 2x10⁶ cells
  • Form
  • Frozen cells
  • Culture Medium
  • DMEM, 10% FBS, 1 μg/mL puromycin
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • Glucagon like peptide 1 receptor
  • Introduction
  • This gene encodes a 7-transmembrane protein that functions as a receptor for glucagon-like peptide 1 (GLP-1) hormone, which stimulates glucose-induced insulin secretion. This receptor, which functions at the cell surface, becomes internalized in response to GLP-1 and GLP-1 analogs, and it plays an important role in the signaling cascades leading to insulin secretion. It also displays neuroprotective effects in animal models. Polymorphisms in this gene are associated with diabetes. The protein is an important drug target for the treatment of type 2 diabetes and stroke. Alternative splicing of this gene results in multiple transcript variants.
  • GPCR Signaling Pathway
  • The endogenous ligand is glucagon-like peptide 1. Targeted protein activation can cause binding of Gs to Go protein which, in turn, cause an inhibition of adenylate cyclase and then decrease of cAMP concentration.
  • Endogenous Ligand
  • Glucagon-like peptide 1
  • Alternative Names
  • GLP-1; GLP-1R; GLP-1-R;glucagon-like peptide 1 receptor;GLP-1 receptor;GLP1 receptor;seven transmembrane helix receptor;GLP1R

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