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MemDX™ Mouse CD40LG NIH3T3 Cell Line (CAT#: S01YF-0123-KX261)

Datasheet
This product is a NIH3T3 stable cell line. It stably expresses Mouse CD40LG to provide cell models for research and screening applications.

Product Information

  • Target Protein
  • CD40LG
  • Target Protein Species
  • Mouse
  • Accession Number
  • NM_011616.2
  • Protein Tag
  • Tag-free
  • Host Cell Type
  • NIH3T3
  • Target Classification
  • CD
  • Target Family
  • CD
  • Target Research Area
  • Autoimmune Research; Immunology Research
  • Related Diseases
  • Immunodeficiency With Hyper-Igm

Product Properties

  • Morphology
  • Fibroblastoid cells growing as a monolayer
  • Assay Types
  • Drug screening and biological assays
  • Resistance
  • Puromycin
  • Mycoplasma Testing
  • Negative
  • Sterility Testing
  • 10 passages
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 5x10⁶ cells
  • Form
  • Frozen cells
  • Freeze Medium
  • 70% DMEM + 20% FBS + 10% DMSO
  • Culture Medium
  • DMEM + 10% FBS + 0.5µg/mL Puromycin
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • CD40 ligand
  • Introduction
  • Predicted to enable CD40 receptor binding activity and integrin binding activity. Involved in isotype switching. Acts upstream of or within B cell differentiation and regulation of immunoglobulin production. Located in external side of plasma membrane. Is expressed in Meckel's cartilage; dorsal root ganglion; and limb. Used to study inflammatory bowel disease and systemic lupus erythematosus. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; artery disease (multiple); autoimmune disease (multiple); combined T cell and B cell immunodeficiency (multiple); and hematologic cancer (multiple). Orthologous to human CD40LG (CD40 ligand).
  • Alternative Names
  • IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor ligand 8b; tumor necrosis factor ligand superfamily member 5

Case Studies

Customer reviews and Q&As    

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