Samples are prepared, denatured, and glycans enzymatically released (e.g., N-glycans with PNGase F; O-glycans via β-elimination) to preserve integrity.
Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsIn the face of formidable hurdles in biopharmaceutical development—from prolonged drug development cycles to intricate challenges in protein expression, purification, and clinical trials—Creative Biolabs delivers advanced Glycan Analysis services. We empower you to significantly accelerate drug discovery, secure high-quality recombinant proteins, and engineer highly specific antibodies by expertly unraveling the complex world of glycans through cutting-edge high-throughput screening platforms and innovative analytical techniques.
Glycosylation, the enzymatic addition of glycans (sugar chains) to proteins and lipids, is one of the most common and crucial post-translational modifications. These modifications profoundly impact the function, efficacy, stability, pharmacokinetics, and immunogenicity of therapeutic proteins, including monoclonal antibodies (mAbs) and other biopharmaceuticals. Analyzing these complex "sugar codes" is essential for understanding biological processes, developing effective drugs, and ensuring product safety. Aberrant glycosylation patterns are also linked to various diseases, including cancer and autoimmune disorders, making precise glycan analysis vital for biomarker discovery and diagnostic development. Without detailed glycan profiling, the full potential and safety profile of many advanced therapeutics, including those potentially interacting with gene therapy products, cannot be fully realized.
Fig.1 Analyzing IgG Fab and Fc glycosylation workflow.1
Addressing the complexities and variability of glycosylation, a critical challenge in biopharmaceutical development, Creative Biolabs' Glycan Analysis service offers a robust and comprehensive solution for characterizing these intricate glycan structures essential to your projects. Adhering strictly to the highest industry standards and employing high-sensitivity, high-specificity testing principles, our service provides specific deliverables, clear solutions, and key problem-solving capabilities. From meticulous sample preparation and advanced high-resolution analysis to in-depth bioinformatic interpretation, our streamlined process typically delivers results within 3 to 8 weeks, ensuring unparalleled quality, accuracy, and reproducibility to optimize your drug development pipeline.
| N-Glycan Analysis |
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N-linked oligosaccharide is covalently bonded with the nitrogen of an asparagine residue, and all N-linked proteins share a basic core structure of conserved pentasaccharide (GlcNAc2Man3) backbone. For immunoglobulins, N-linked oligosaccharides have received particular attention because changes in the attached glycans can impact immunoglobulin solubility, structural stability, and biological functions. For example, the differential of a single monosaccharide at Asn-297 glyco-site at the Fc fragment of human IgG can drastically affect IgG binding to FcγR and complement action. Technologies for N-Glycan analysis provided by Creative Biolabs, including but not limited to:
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| O-Glycan Analysis |
O-acetylgalactosamine (O-GalNAc) and O-acetylglucosamine (O-GlcNAc) are the common O-glycosylation types, among which, O-GalNAc is attached to the hydroxyl group of the protein serine or threonine residues through an α-linkage, while O-GlcNAc is attached through a β-linkage. Technologies for O-Glycan analysis provided by Creative Biolabs, including but not limited to:
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Samples are prepared, denatured, and glycans enzymatically released (e.g., N-glycans with PNGase F; O-glycans via β-elimination) to preserve integrity.
Released glycans can be fluorescently labeled for enhanced detection.
Techniques like HILIC or SPE enrich and desalt glycans, removing contaminants for high-resolution analysis.
Advanced platforms (HPAEC-PAD, HPLC-FLD/MS, MALDI-TOF MS, LC-MS/MS) provide detailed structural elucidation and quantitative profiling.
Raw data is processed via bioinformatic analysis, including automated scripts, comparative plots, and heatmaps, for glycoform identification and quantification.
"The precision and detail using Creative Biolabs' Glycan Analysis in our mAb research has significantly improved our understanding of immunogenicity, directly guiding our lead candidate selection."
— 2 months ago, An***rew M.
"We found the high-throughput capability of Creative Biolabs' Glycan Analysis invaluable for screening our glycoengineered cell lines, greatly facilitating our bioproduction optimization efforts."
— 4 weeks ago, Dr. S***ara J.
"Creative Biolabs' team provided invaluable insights into O-glycan structures, which were crucial for validating the stability and activity of our therapeutic glycoprotein. Their expertise truly made a difference."
— 3 months ago, Pr***sor L.
A1: We accept a wide range of samples, including purified glycoproteins, monoclonal antibodies, serum, plasma, cell lines, and tissues. The specific sample volume and preparation requirements may vary depending on the chosen analytical technique, so we always recommend discussing your project with our experts for detailed guidance.
A2: While general protein characterization focuses on amino acid sequence and basic structural integrity, Glycan Analysis specifically delves into the complex sugar modifications attached to proteins. These glycans are non-template driven, meaning they're not directly coded by genes, yet they are crucial for a protein's proper folding, stability, biological activity, and interaction with other molecules. For biopharmaceuticals, precise glycan profiling is absolutely critical to ensure optimal efficacy, safety, and consistent quality, especially when compared to protein-only characterization.
A3: Absolutely. Aberrant glycosylation is a hallmark of many diseases. Our advanced Glycan Analysis can profile altered glycan patterns on cell surfaces or in biofluids, helping to identify disease-specific glycan biomarkers or changes that correlate with disease progression or therapeutic response. This supports diagnostic development and more targeted therapeutic strategies.
Creative Biolabs is your trusted partner for cutting-edge Glycan Analysis, driving innovation in biopharmaceutical research and development. Our comprehensive services, backed by extensive expertise and advanced platforms, provide the detailed insights you need to understand, characterize, and optimize the glycosylation of your therapeutic molecules. Please feel free to get in touch with us.
Reference
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