Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications. Highlights
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsCreative Biolabs has established a quantitative platform for screening antibody libraries using bacterial display systems and fluorescence-activated cell sorting (FACS) technology. Utilizing our platform, clones with high-affinity to fluorescently-labeled hapten can be isolated with the principles of mutation and selection. In combination with FACS, antibody affinity maturation by bacterial display technology has become a powerful tool for rapid isolation and characterization of desirable mutants from libraries.
Affinity maturation is the process of improving the affinity of antibody for an antigen. Normally, these antibodies undergo affinity maturation in vivo, but sometimes the required affinity may be higher than the affinity of the leads obtained, thus requiring further affinity maturation in vitro, which can be obtained in the laboratory by mutation and selection. Creative Biolabs has extensive experience in antibody affinity maturation. We usually use scFv as the antibody form in affinity maturation. In addition, we also provide affinity maturation services for single domain antibodies. Creative Biolabs' bacterial display-based affinity maturation service has successfully been used to isolate high-affinity proteins, including antibodies, for our worldwide clients.
The anchored periplasmic expression system (APEx) has been shown to be a multifunctional technique for isolating high affinity antibodies from libraries expressed in E. coli and has been successfully used for the display and affinity maturation of antibody fragments and full-length antibodies. A typical round of basic APEx procedure for antibody affinity maturation includes the following steps.
1. Spheroplast displaying antibodies are prepared by disrupting the outer membrane of E. coli, and the spheroplast is labeled with fluorescent antigens.
2. Strong antigen-bound spheroplasts are sorted and harvested using a flow cytometer sorter.
3. The genes of the antibodies are rescued from the harvested spheroplasts, and finally, the genes of the antibody are cloned.
Researchers typically construct a random mutant library of original antibody fragments by error-prone PCR and combine the library with several rounds of panning and/or flow cytometry sorting to achieve improved antibody affinity. The larger the antibody library, the greater the likelihood of obtaining high affinity antibodies.
The bacterial antibody display library can be coupled with FACS to select high-affinity antibodies that specifically bind to the targeted antigen, allowing a more controllable and quantitative selection process. Creative Biolabs has extensive experience in the field of in vitro display. With our advanced platforms and extensive experience, we are confident in offering highly efficient services for antibody affinity maturation and guaranteeing the finest results for our customers all over the world. Please do not hesitate to contact us for more details.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
