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MemDX™ Cell-free Membrane Protein Expression in Wheat Germ lysate for protein expression screening (CAT#: MPCFK-3-2)

Datasheet
MemDX™ Cell-free Protein Expression in Wheat Germ lysate for protein expression screening Kit is a cell-free protein synthesis system using wheat germ extracts for protein production. By combining the transcription and translation reactions to be performed at the same time in the same reaction mixture, protein expression reactions can easily be set up with few pipetting steps. It's optimal for doing small-scale protein expression screening experiments. The kit contains a small amount of labeled lysine that allows to prepare a fluorescently labeled protein for protein expression analysis.

Product Specifications

  • Lysate Type
  • Wheat Germ lysate
  • Size
  • 24 reactions
  • Reaction Volume
  • 55 µL per reaction
  • Expected Yield of Positive Control
  • 20 µg/ml
  • Components
  • Cloning Vector
    Control Vector
    Wheat Germ Extracts
    Reaction buffer
    SPU PCR sense primer
    SP6 PCR sense primer
    Labeled lysine tRNA
  • Storage Conditions
  • Store at -80°C.

Case Studies

Customer reviews and Q&As    

Customer Review

The kit is extremely simple to operate)

Transcription and translation were performed in the same reaction mixture with very simple steps)
2023.1.09)

Q&As

What are the advantages of the wheat germ cell-free expression system?)

The advantages of wheat germ cell-free expression systems are: well-established systems, best yields in eukaryotic systems after multiple protein tests, have very high solubility rates, have been shown to synthesize very large proteins (≈200 kDa), usually do not require codon optimization, have stable systems allowing the use of different additives.)
2023.01.16)

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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