100 N-Glycan Microarray

N-glycan is one of the most common modes of glycosylation in the human body. Within the immune system, the N-linked glycans on an immune cell surface will help dictate that migration pattern of the cell. The glycosylation patterns on various immunoglobulins bestow them with unique effector functions. N-glycans may also be involved in cell recognition, which may be relevant to the pathophysiology of various autoimmune diseases. Due to this important effect in signal transduction, the interactions between proteins and N-glycans attract more attention in glycoprotein field. As a world-leading service provider in the field of microarrays, Creative Biolabs offers comprehensive N-glycan microarray service to global clients. We guarantee to provide excellent service and competitive products to customers all over the world.

Introduction of 100 N-Glycan Microarray

N-linked glycosylation is the attachment of an oligosaccharide consisting of several sugar molecules to a nitrogen atom, usually pertaining to an asparagine residue of a protein. This type of linkage is important for both the structure and function of many eukaryotic proteins, while it is very rare in bacteria. The nature of N-linked glycans attached to a glycoprotein is determined by the protein and the cell in which it is expressed and often show different bioactivities. Our 100 N-glycan microarray slides are specially coated to be capable of immobilizing natural N-glycans with close-ring structure at their reducing end (GlcNAc), which acts as the core polysaccharide. 100 N-glycan microarray is used as a general test to help researchers determine binding characteristics of proteins or other potential biological samples to an array of 100 fundamental N-glycans.

Fig.1 Typical structures of N-glycan and 14-sugar precursor. (Lyons, 2015)

Applications of 100 N-Glycan Microarray

Changes in N-linked glycosylation have been associated with different diseases including rheumatoid arthritis, type 1 diabetes, Crohn's disease, and cancers, while many therapeutic proteins in the market are N-glycosylated antibodies. Moreover, N-linked oligosaccharides, in particular, play a major role in intercellular interactions and immune cell functions, making them often preferred glycan candidates for the study of carbohydrate-binding-vaccines. The fundamental N-glycan structures provided in this microarray can provide an understanding of basal binding determinants for antibodies or other proteins of interest. Furtherly, Creative Biolabs offers more services based on this technology, such as glyco-based vaccine development, Anti-Glycoprotein Antibody Development and so on.

The research of N-glycan is popular in therapeutic antibody development due to its irreplaceable role in the immune system. We have successfully developed many glycan microassay products and corresponding testing services to accelerate your program. For more details, please feel free to contact us.

Reference

1. Lyons, J.J.; et al. Glycans instructing immunity: the emerging role of altered glycosylation in clinical immunology. Frontiers in pediatrics. 2015, 3, 54.

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