Human Milk Oligosaccharide Microarray

Human milk oligosaccharides (HMOs) are the most abundant carbohydrate component of breastmilk after lactose, and are composed of a heterogeneous mix of at least 200 distinct glycan structures. These glycans are unique to human milk, which can protect newborns from diseases and infections, build healthy intestinal bacteria, and are essential for the development of the baby's brain, intestines and immune system. Creative Biolabs provides high-quality products and customized services for the analysis of HMO-proteins interactions. We have successfully developed many human milk oligosaccharides microassay products and furtherly extent the application of this platform by combined with other support technologies. We guarantee to provide first-class products and excellent service to customers all over the world.

Fig.1 An example of the structural diversity of human milk oligosaccharides. (Smilowitz, 2014)

Introduction of Human Milk Oligosaccharide Microarray

The structural complexity of HMOs ranges from relatively simple glycans, such as lactose and sialyl- and fucosyl-lactose, to highly complex, multi-branched structures having different appendages at each branching point.

It currently is believed that HMOs can function both as prebiotics and as decoy receptors that inhibit the attachment of pathogenic microorganisms to the colonic mucosa. Given the importance of HMOs to infant health, there is currently considerable interest in identifying their protein receptors in humans, as well as those associated with viral and bacterial pathogens. However, virtually nothing is known about the importance of the molecular complexity of HMOs for binding and biological activity due to the difficulty to obtain the determined structural HMOs. However, the HMO microarrays provide a sensitive and high-throughput approach to HMO-binding proteins screening which uncover the binding selectivity controlled by glycan complexity.

Applications of Human Milk Oligosaccharide Microarray

Fig.2 HMOs may benefit the breast-fed infant in multiple different ways. (Bode, 2012)

Except for its important effect on the immune system, recent studies have furthermore shown the beneficial properties of HMOs extending to protection against infant-associated gastrointestinal conditions with the binding ability of intestinal bacteria. Therefore, the screen of HMO-binding proteins ranges from endogenous protein to microorganism.

A novel study has shown the application of human milk oligosaccharide microarray technology. An HMO microassay chip containing more than fifty kinds of HMO was developed to detect the interactions between proteins and HMOs. The result uncovered that complex architectures of glycans can influence binding selectivity in unanticipated manners. Similarly, our HMO microassay also contains a series of classic glycan epitopes that have been widely reported. This product can accelerate the research of HMO and its interaction.

Because of the functional diversity, HMO-related research appears on high IF journals constantly. Creative Biolabs provides the most comprehensive Human Milk Oligosaccharide Microarray to meet your requirement. For more details, please feel free to contact us.

References

1. Smilowitz, J.T.; et al. Breast milk oligosaccharides: structure-function relationships in the neonate. Annual review of nutrition. 2014, 34, 143-169.
2. Bode, L.Human milk oligosaccharides: every baby needs a sugar mama. Glycobiology. 2012, 22(9), 1147-1162.

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