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MemDX™ Human FLAG tagged AGTR1 HEK293T Cell Line, Calcium flux assay (CAT#: S01YF-1022-KX203)

Datasheet
This product is a HEK293T stable cell line. It stably expresses FLAG tagged AGTR1 to provide cell models for research and screening applications.

Product Information

  • Target Protein
  • AGTR1
  • Target Protein Species
  • Human
  • Accession Number
  • NM_000685.4
  • Protein Tag
  • FLAG-tag at N-terminus
  • Host Cell Type
  • HEK293T
  • Target Classification
  • GPCR
  • Target Family
  • Angiotensin
  • Target Research Area
  • Cardiovascular Research; Digestive and Renal Research; Infectious Research
  • Related Diseases
  • Renal Tubular Dysgenesis; Hypertension

Product Properties

  • Assay Types
  • Calcium flux assay
  • Resistance
  • Puromycin
  • Stability
  • Stable for a minimum of 2 months in continuous culture
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 2x10⁶ cells
  • Form
  • Frozen cells
  • Culture Medium
  • DMEM, 10% FBS, 1 μg/mL puromycin
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • Angiotensin II receptor type 1
  • Introduction
  • Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Alternative splicing of this gene results in multiple transcript variants.
  • GPCR Signaling Pathway
  • The endogenous ligand is angiotensin II. Targeted protein activation can cause binding of Gq to G11 protein which, in turn, activate a phosphatidylinositol-calcium second messenger system.
  • G coupling
  • Gq
  • Endogenous Ligand
  • Angiotensin II
  • Alternative Names
  • AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B;type-1 angiotensin II receptor;type-1B angiotensin II receptor;Angiotensin II type-1 receptor;AGTR1;AGTR1A; AT2R1B

Case Studies

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