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Magic™ Human CLDN6 B16F10 Cell Line (CAT#: S01YF-0123-KX102)

Datasheet
This product is a B16F10 stable cell line. It stably expresses Human CLDN6 to provide cell models for research and screening applications.

Product Information

  • Target Protein
  • CLDN6
  • Target Protein Species
  • Human
  • Accession Number
  • NM_021195.5
  • Protein Tag
  • Tag-free
  • Host Cell Type
  • B16F10
  • Target Classification
  • Others
  • Target Family
  • Others
  • Target Research Area
  • Infectious Research
  • Related Diseases
  • Hepatitis C Virus

Product Properties

  • Morphology
  • Epithelial
  • Assay Types
  • Drug screening and biological assays
  • Resistance
  • Puromycin
  • Mycoplasma Testing
  • Negative
  • Sterility Testing
  • 10 passages
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 5x10⁶ cells
  • Form
  • Frozen cells
  • Freeze Medium
  • 70% McCoy's 5a + 20% FBS + 10% DMSO
  • Culture Medium
  • McCoy's 5a + 10% FBS + 1µg/mL Puromycin
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • Claudin 6
  • Introduction
  • Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This gene encodes a component of tight junction strands, which is a member of the claudin family. The protein is an integral membrane protein and is one of the entry cofactors for hepatitis C virus. The gene methylation may be involved in esophageal tumorigenesis. This gene is adjacent to another family member CLDN9 on chromosome 16.
  • Alternative Names
  • CLDN6; claudin-6; skullin; Claudin 6

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