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HighlightsIntroduction of MFSD2B
MFSD2B is an orphan Major Facilitator Superfamily (MFS) transporter with an estimated 12 transmembrane domains. It is located in the plasma membrane and in the inner membrane of the cell. MFSD2B protein is present in the bone marrow and spleen but not in the kidneys and liver. In the blood, MFSD2B protein is significantly expressed in RBC and platelets. MFSD2B is present in the RBC ghost membrane and is sensitive to some trypsin digestion, indicating its plasma membrane localization. Besides, MFSD2B expression is increased in young RBCs, bone marrow, and spleen in 5-fluorouracil (5FU)-treated mice. The MFSD2B transcript is mainly present in the erythroid lineage, producing red blood cells and platelets, but not in the lymphoid lineage. Mouse MFSD2B is also a member of the Major Facilitator Superfamily (MFS) of transporters with 494 amino acid residues. The MFSD2B cDNA has been reported in various mammals. Human MFSD2B showed 83% identity and 96% similarity to mouse MFSD2B. The molecular weight of both human and mouse MFSD2B are detected to be approximate 43 kDa in size.
| Basic Information of MFSD2B | |
| Protein Name | Major facilitator superfamily domain-containing protein 2B |
| Gene Name | MFSD2B |
| Aliases | hMfsd2b |
| Organism | Homo sapiens (Human) |
| UniProt ID | A6NFX1 |
| Transmembrane Times | 12 |
| Length (aa) | 504 |
| Sequence | MAAPPAPAAKGSPQPEPHAPEPGPGSAKRGREDSRAGRLSFCTKVCYGIGGVPNQIASSATAFYLQLFLLDIAQIPAAQVSLVLFGGKVSGAAADPVAGFFINRSQRTGSGRLMPWVLGCTPFIALAYFFLWFLPPFTSLRGLWYTTFYCLFQALATFFQVPYTALTMLLTPCPRERDSATAYRMTVEMAGTLMGATVHGLIVSGAHRPHRCEATATPGPVTVSPNAAHLYCIAAAVVVVTYPVCISLLCLGVKERPDPSAPASGPGLSFLAGLSLTTRHPPYLKLVISFLFISAAVQVEQSYLVLFCTHASQLHDHVQGLVLTVLVSAVLSTPLWEWVLQRFGKKTSAFGIFAMVPFAILLAAVPTAPVAYVVAFVSGVSIAVSLLLPWSMLPDVVDDFQLQHRHGPGLETIFYSSYVFFTKLSGACALGISTLSLEFSGYKAGVCKQAEEVVVTLKVLIGAVPTCMILAGLCILMVGSTPKTPSRDASSRLSLRRRTSYSLA |
Function of MFSD2B Membrane Protein
Sphingosine-1-phosphate (S1P), a potent signaling lipid secreted by red blood cells and platelets, plays numerous biologically significant roles. The Major facilitator superfamily transporter 2b (MFSD2B) is essential for S1P export from red blood cells and platelets. The S1P export conducted by MFSD2B is not driven by the Na+ or K+ ion gradient, although the alanine substitution of Asp85 alters the S1P transport of MFSD2B. Comprehensive lipidomics analysis showed significant and specific accumulation of S1P species in Mfsd2b knockout red blood cells and platelets compared to wild-type controls. Similarly, biochemical assays in knocked out red blood cells, platelets, and cell lines with over-expressed human and mouse Mfsd2b proteins demonstrated that MFSD2B can actively output S1P. Plasma S1P levels in knockout mice are significantly reduced by 42-54% compared to wild type, indicating that the MFSD2B pathway contributes to approximately half of the plasma S1P pool. The reduction in plasma S1P in knockout mice is not sufficient to cause vascular leakage, but it can make mice more sensitive to anaphylactic shock.
Fig.1 Plasma S1P is mainly contributed by three major cell types: red blood cells, platelets, and endothelial cells. (Ashok, 2017)
Application of MFSD2B Membrane Protein in Literature
This article finds that knockdown of MFSD2B in MEDEP-E14 cells can reduce the S1P export of the cells, indicating that MFSD2B is a novel S1P transporter in erythroid cells.
This paper demonstrates that S1P secretion by Mfsd2b is critical for red blood cell morphology.
This article shows that targeting S1P production in a tumor and the host would help reduce both growth and metastasis.
MFSD2B Preparation Options
Membrane protein research has made significant progress over the past few years. Based on our versatile Magic™ membrane protein production platform, we could offer a range of membrane protein preparation services for worldwide customers in reconstitution forms and multiple active formats. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-MFSD2B antibody development services.
In the past years, Creative Biolabs has successfully produced many functional membrane proteins for our global customers. We are pleased to accelerate the development of our clients’ programs through our one-stop, custom-oriented service. For more detailed information, please feel free to contact us .
Reference
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