Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsThe inherent complexities of the drug discovery process, including prolonged development timelines, imprecise protein target identification, and the imperative for interaction validation, represent significant challenges within the biopharmaceutical sector. At Creative Biolabs, we are confident in unlocking hidden protein interactions and accelerating your research with our photoaffinity labeling expertise. Our PAL service helps you accelerate drug discovery by precisely and unequivocally identifying and validating small molecule-protein interactions. We achieve this through the application of advanced photoaffinity labeling technology combined with cutting-edge mass spectrometry platforms, offering a robust and reliable solution for target deconvolution and mechanism of action studies.
PAL is a powerful biochemical proteomics technique used to investigate and map the interactions between a small molecule and its biological targets directly within a native cellular environment. Unlike traditional affinity chromatography, which can suffer from non-specific interactions, PAL employs a photoreactive probe that, upon exposure to ultraviolet (UV) light, forms a stable covalent bond with nearby proteins. This "capture" method allows for the robust identification of both high-affinity and transient interactions, offering a more accurate snapshot of a compound's true protein targets.
This technique is particularly valuable in drug discovery as it can deconvolute the targets of bioactive molecules discovered through phenotypic screens, where the mechanism of action is unknown. By covalently tagging the binding partners, PAL provides a direct link between a small molecule's biological effect and its molecular targets. The integration of PAL with advanced mass spectrometry has transformed the field, enabling the comprehensive and quantitative analysis of the entire cellular proteome, leading to the precise screening and identification of drug targets, which is critical for understanding efficacy and toxicity.
Fig.1 PAL for the detection of protein targets.1
Identify unknown protein targets: Use a photoaffinity probe based on a small molecule of interest (e.g., a drug candidate) to "fish" for its unknown protein targets in a complex biological mixture.
Map binding sites: By using a photoaffinity probe, you can identify the specific amino acid residues on a protein that are in close proximity to the ligand's binding site.
Investigate protein-protein interactions (PPIs): PAL can be used to capture transient or weak PPIs by incorporating a photoreactive unnatural amino acid into one of the proteins.
Elucidate drug mechanisms of action: By labeling the actual target of a drug, you can gain a deeper understanding of how the drug works.
Our Photoaffinity Labeling Service provides a clear path to identifying the on- and off-targets of your small molecule drug candidates. This service is designed to deliver unambiguous, high-confidence data, empowering you to make informed decisions and accelerate your research pipeline. We provide a definitive solution for target deconvolution, even in complex biological systems, allowing you to move from phenotypic screens to validated drug-target pairs with efficiency and confidence.
Custom-synthesize a photoaffinity probe by incorporating a photoreactive group (e.g., diazirine or benzophenone) with/without a reporter tag onto your small molecule. This stage includes rigorous internal validation to ensure the probe retains the parent compound's biological activity and exhibits optimal photoreactivity.
The optimized probe is incubated with the provided biological samples (mainly cultured cells or cell lysis). The reaction system is then exposed to UV light, which triggers the photoreactive group to form a stable covalent bond with proteins in close proximity. This process effectively "traps" non-covalent interactions, and can be executed in live cells to preserve native protein-protein interactions and conformational states.
Subsequent to photocross-linking, the samples are lysed, and the probe-labeled proteins are enriched via affinity chromatography. This step efficiently isolates the targets from the complex cellular proteome.
The enriched protein complexes are enzymatically digested into peptides, which are then analyzed using state-of-the-art high-resolution mass spectrometry. This procedure facilitates the accurate identification of proteins that were covalently labeled by the probe.
The raw mass spectrometry data will be exported and analyzed to generate a comprehensive report detailing the identified protein targets, their relative abundance, and the confidence level for each interaction. This includes comparative analysis between treated and control groups to mitigate non-specific binding.
We offer a complete, end-to-end PAL service that covers every phase of your project. From the initial photoaffinity probe design and synthesis to in vitro/in-cell target enrichment and labeling, and finally to mass spectrometry analysis and data interpretation.
Our PAL services are applicable to purified proteins, cell lysates, live cells, supporting multiple species and complex biological matrices.
Beyond validating known targets, our PAL service excels at identifying new targets and potential off-target effects within complex biological systems.
Our PAL services are highly customized to meet your specific needs. Whether you're targeting a specific protein for screening or exploring a complex signaling pathway, we will work closely with you to develop the most suitable experimental strategy.
Our workflow is designed for transparency and is structured to provide a comprehensive, step-by-step approach to small molecule-target interaction analysis. To provide comprehensive and high-quality PAL services, we kindly ask you to provide detailed information about your project. Specifically, please include:
Providing this information will help us prepare a tailored formal quotation and PAL workflow and ensure optimal data quality for your study.
Creative Biolabs stands apart as a leader in interaction screening and analysis, distinguished by our robust technological platforms, profound scientific expertise, and commitment to delivering actionable insights. The foundation of our services rests upon rigorous scientific principles and a dedicated focus on accelerating client success. To further support your drug discovery and development efforts, we offer a series of Biomolecular Interaction services designed to provide an end-to-end solution, including but not limited to:
| Yeast two-hybrid (Y2H) | TurboID |
| Protein-fragment complementation assay (PCA) | Yeast one-hybrid (Y1H) |
| Bimolecular fluorescence complementation (BiFC) | Bioluminescence resonance energy transfer (BRET) |
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
