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MemDX™ Rat FLAG tagged P2RY12 CHO dhfr- Cell Line, Calcium flux assay (CAT#: S01YF-1022-KX579)

Datasheet
This product is a CHO dhfr- stable cell line. It stably expresses FLAG tagged P2RY12 to provide cell models for research and screening applications.

Product Information

  • Target Protein
  • P2RY12
  • Target Protein Species
  • Rat
  • Accession Number
  • N<_022800
  • Host Cell Type
  • CHO dhfr-
  • Target Classification
  • GPCR
  • Target Family
  • Purinergic
  • Target Research Area
  • Cardiovascular Research
  • Related Diseases
  • Bleeding Disorder; Nizon-Isidor Syndrome

Product Properties

  • Assay Types
  • Calcium flux assay
  • Resistance
  • Hygromycin
  • Stability
  • Stable for a minimum of 2 months in continuous culture
  • Mycoplasma Testing
  • Negative
  • Biosafety Level
  • Level 1
  • Activity
  • Yes
  • Quantity
  • 2x10⁶ cells
  • Form
  • Frozen cells
  • Culture Medium
  • α-MEM, 10% FBS, 100 μg/ml hygromycin
  • Selective Antibiotic(s)
  • Regular antibiotics active against mycoplasmas, bacteria and fungi.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Incubation
  • 37°C with 5% CO₂
  • Applications
  • Drug screening and biological assays
  • Application Notes
  • Cells were plated in a 384-well plate and incubated overnight at 37°C and 5% CO₂ to allow the cells to attach and grow. Cells were then stimulated with a control for high-throughput drugs screening andfunctional assays.
  • Use Restrictions
  • These cells are distributed for research use only.
  • Shipping
  • Dry ice
  • Storage
  • Liquid nitrogen

Target

  • Full Name
  • Purinergic receptor P2Y12
  • Introduction
  • Enables G protein-coupled ADP receptor activity. Involved in several processes, including G protein-coupled receptor signaling pathway; inorganic cation transmembrane transport; and intracellular signal transduction. Located in basal plasma membrane; caveola; and external side of plasma membrane. Colocalizes with mitochondrion. Used to study abdominal aortic aneurysm and thrombosis. Human ortholog(s) of this gene implicated in asthma; cerebrovascular disease; peripheral artery disease; platelet-type bleeding disorder 8; and type 2 diabetes mellitus. Orthologous to human P2RY12 (purinergic receptor P2Y12).
  • GPCR Signaling Pathway
  • The endogenous ligand is Purinergic. Targeted protein activation can cause binding of Gi to Go protein which, in turn, cause an inhibition of adenylate cyclase and then decrease of cAMP concentration.
  • G coupling
  • Gi & Go
  • Endogenous Ligand
  • Purinergic
  • Alternative Names
  • P2y12;P2Y purinoceptor 12;P2Y12 platelet ADP receptor;purinergic receptor P2Y, G-protein coupled, 12;P2ry12

Case Studies

Customer reviews and Q&As    

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