Mesenchymal Stem Cells (MSCs) Isolation and Expansion Services

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Mesenchymal Stem Cells (MSCs) Isolation and Expansion Services

Mesenchymal stem/stromal cells (MSCs) are promising candidates for use in cell-based therapies and therefore have attracted significant interest. However, the development of their clinical utility relies on reliable sources of MSCs, defined isolation and expansion protocols. To facilitate the research in this field, Creative Biolabs provides high-quality isolation and expansion services of MSCs that are derived from a wide range of tissues and sources.

MSCs Isolation & Expansion

MSCs can be derived from different sources and can be isolated from various tissues, such as umbilical cord, bone marrow, cartilage, muscle, and adipose tissues. Besides, fetal dermis tissues, various dental tissues, pancreatic tissue, breast milk, menstrual blood, and endometrium have been reported to contain MSCs as well. Additionally, the MSC pool of a tissue may be expanded by dedifferentiation of differentiated cells under certain conditions. Cells from different tissues differ in properties including cell surface markers expressed and differentiation abilities. Even populations derived from a single source may be heterogeneous and have different developmental potentials.

Creative Biolabs employs different protocols for MSC isolation.

  • Firstly, the adherence properties of MSCs can be utilized for simple isolation of MSCs. MSCs are obtained by seeding the bone marrow cells on tissue plastic dishes, followed by washing out of the non-adherent cells.
  • Secondly, the extraction of the mononuclear cells from the bone marrow through density gradient centrifugation or the enzymatic treatment of solid tissues with collagenase can be used to increase the yield of MSCs.
  • Besides, the immunoisolation and immunodepletion techniques based on different surface markers, in combination with fluorescence-activated cell sorting or magnetic beads, can improve the purity of the resulted MSCs yields.

MSCs represent a rare population in bone marrow and other tissues and thus ex-vivo expansion and manipulation are required to reach a sufficiently large scale. The most important factor of successful expansion of MSCs is the culture conditions. Creative Biolabs uses optimized and standardized complete mediums for the reproducible and reliable generation and expansion of MSCs derived from different sources. The yield and the quality of the expanded cells are both ensured.

Sources of MSCs. (Dittmer, 2011) Fig.1 Sources of MSCs. (Dittmer, 2011)

Bone Marrow (BM)-derived MSCs

Among the available sources, BM is the most commonly used one. MSCs from bone marrow are the main cell source for tissue repair and engineering, and vehicles of cell-based gene therapy. Creative Biolabs has established standardized, reliable, and easy-to-perform protocols for isolation and culture of BM-MSCs. Using our protocol, the isolated BM-MSCs exhibit multiple lineage differentiation potentials.

Blood-derived MSCs

Blood has become a very attractive potential source of stem cells to be studied, based on their differentiation capacity and therapeutic potential. Creative Biolabs offers MSCs isolation from peripheral blood, umbilical cord blood, and menstrual blood.

Placental- and Umbilical Tissue-derived MSCs

The placent and umbilical tissues can be easily attained as promising sources of stem cells. These populations of MSCs have advantages such as greater proliferative capacity and immunosuppressive properties than maternal MSC populations.

Adipose-derived MSCs

Because of their easy isolation and relative abundance, adipose tissues have become a reliable source of MSCs. These cells have multiple mesodermal lineage differentiation potentials in vitro, evidenced by adipogenic, osteogenic, and chondrogenic phenotype display when cultured in the presence of established lineage-specific differentiation factors. Besides, they can also differentiate into nervous tissues, hepatic lineage, and cardiomyocytes.

Other Tissues-derived MSCs

MSCs can also be isolated from other tissues, such as muscles, cartilage, dental pulp, and dermis tissues. We can customize the culturing conditions and protocols to maximally expand the MSCs derived from sources while maintaining the multipotency.

Features

Creative Biolabs employs different protocols for MSC isolation.

  • Long-term viability, multipotent potential, integrity and uniformity of your cells are ensured
  • Amenable to the research of different cellular applications and assay endpoints
  • Sufficient cell populations with the desired functionality and phenotype
  • Ongoing technical support and a range of tailored media with specific supplementation to ensure the optimal culture
  • Customized services and individualized support to suit specific research requirements

Creative Biolabs stands behind the high quality of our services and products. We can precisely cater to your specific requirements with our flexible, custom MSC isolation and expansion services. If you have any questions, please contact us.

Reference

  1. Dittmer, J.; et al. Involvement of mesenchymal stem cells in breast cancer progression. Breast Cancer-Focusing Tumor Microenvironment, Stem Cells and Metastasis. 2011.