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CSF3R

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All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

Background

CSF3R is an 813-amino acid type I transmembrane glycoprotein of the hematopoietin/cytokine receptor superfamily with an estimated molecular weight from 90–130 kDa based on glycosylation state. The human G-CSF/CSF3R complex recently solved by crystallography at 2.8 Å has a distinct 2:2 stoichiometry and cross-over interactions between the Ig-like domains, but such signalling architecture resembles that of the IL-6/gp130 signaling complex more than it does with similarity to murine complexes. Such a structural understanding has important ramifications in the design of therapeutic G-CSF and development of drug(s) targeting the receptor. CSF3R signaling is required for basal neutrophil production under homeostatic conditions and also essential for emergency granulopoiesis during infection, inflammation and stress. The receptor regulates myeloid progenitor proliferation, differentiation, survival and systemic provision to the peripheral blood. In addition to its canonical hematopoietic functions, CSF3R mediates neuroprotection and tissue repair and is associated with tumor biology since aberrant G-CSF/CSF3R signaling has been shown for instance in solid tumors such as ovarian cancer, bladder cancer and squamous cell carcinoma.

Fig.1 CSF3R-AS promotes hepatocellular carcinoma progression. (OA Literature)Fig.1 Schematic representation of the model.1

CSF3R Protein Function: A Master Regulator of Granulocyte Fate and Myeloid Transformation

This emphasizes the requirement for CSF3R in myeloid cell biology, with functional contributions that span hematopoietic development, innate immunity and oncogenic transformation:

  • Neutrophil Development and Emergency Granulopoiesis: The major receptor for neutrophils origin from hematopoietic stem cell is CSF3R. Only Low-level G-csf signaling maintains stable state granulopoiesis under the basal condition. Upon infection or inflammation, the increased G-CSF levels cause profound and persistent CSF3R activation that induces exhaustive myeloid progenitor expansion, expedited neutrophil maturation, and mobilization of mature neutrophils from marrow stores to peripheral blood circulation. This is important as this emergency response is essential for host defense against bacterial and fungal pathogens.
  • Hematopoietic Stem Cell Mobilization and Transplantation: High-dose G-CSF administration mediates the mobilization of hematopoietic stem and progenitor cells from bone marrow niche to peripheral blood, facilitated by CSF3R in preparation for autologous or allogeneic transplantation. Again, mobilization is through CSF3R signalling in osteoblasts and bone marrow stroma which changes the CXCL12-CXCR4 retention axis leading to release of HSPCs into blood circulation.
  • SCN-Associated Leukemogenesis and Truncating Mutations: Long-term G-CSF therapy selects for acquired mutations in the C-terminal cytoplasmic domain of CSF3R that truncate most of this distal ~100 amino acid region. These truncating mutations mainly modify the pro-apoptotic effects of mutant ELANE results in the clonal expansion of HSPCs through persistent STAT5 activation and increased levels of ROS. CSF3R mutations are detectable years before overt MDS/AML diagnosis :mutational burden correlates with risk of transformation.

CSF3R Membrane Protein Product

Creative Biolabs provides a variety of high-performance CSF3R membrane protein for research applications, critical to gain structural and functional insights into this indispensable hematopoietic cytokine receptor. CSF3R is expressed in multiple expression systems (Virus-Like Particles (VLPs), detergent-solubilized formats, and HEK293-derived mammalian cell membranes) using our proprietary Membrane Protein technology. These proteins have been extensively verified and include Membrane Protein in Virus-Like Particles (MP-VLPs), human/mouse/rat CSF3R expressed in HEK293 or admixtures of specialized hematopoietic-compatible systems for applications such as ELISA, monoclonal & polyclonal antibody generation and characterization, G-CSF binding studies; JAK2 recruitment experiments; and functional myeloid signaling assays. These establish dependable instruments for researchers to investigate CSF3R associations with G-CSF, JAK2, STAT3/5 and downstream signaling effector. Our CSF3R preparations faithfully preserve Ig-fold and CRH domain structure, the integrity of the WSXWS motif and tyrosine phosphorylation sites in a mammalian system sophisticated enough to rationalize whether properties important for therapeutic antibodies or mechanistic leukemia studies need physiological relevance.

CSF3R Protein Product

Not finding the membrane protein product you need? Contact us to start your one-stop custom service!

CSF3R Stable Cell Line Product

Creative Biolabs offers customized CSF3R stable cell lines designed to provide consistent target expression for a range of research applications. These stable cell models can support G-CSF binding studies, CSF3R signaling research, myeloid differentiation studies, compound screening, and other investigations of CSF3R biology and pharmacology. Depending on specific experimental requirements, CSF3R-overexpressing or knockdown cell models may be developed to support gain- or loss-of-function studies. Appropriate expression and functional characterization strategies can be selected according to the intended research application.

CSF3R Stable Cell Line Product

Not finding the stable cell line product you need? Contact us to start your one-stop custom service!

CSF3R Recombinant Antibody Product

We offer a robust portfolio of recombinant antibodies against CSF3R, designed for all your research needs. these monoclonal antibodies are produced by rationally designed recombinant methods and have advantages in specificity, sensitivity and batch-to-batch reproducibility over traditional polyclonal-based antibodies. We validate CSF3R recombinant antibodies for use in WB, ELISA, FCM, IF, ICC, IHC and IP across a variety of samples including bone marrow aspirates; peripheral blood mononuclear cells isolated from the peripheral blood mononuclear cells of leukemia patients and healthy subjects; leukemia cell lysates; formalin-fixed paraffin-embedded (FFPE) tissue sections These include antibodies against different CSF3R specific epitopes: extracellular Ig-like domain, CRH domain and membrane-proximal region for G-CSF competition studies, ligand binding assays, T618I mutation detection; cytoplasmic tail for measuring phosphorylation state—all allowing ample profiling of CSF3R in samples from health versus disease and before vs. after therapeutic intervention.

CSF3R Recombinant Antibody Product

Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!

Product Features

  • High Purity & Activity: High purity and verified integrity for dependable downstream work.
  • Diverse Expression Systems: Choice of expression formats to match routine or specialized projects.
  • Species Reactivity: Products available for human, mouse, pig, and other species.
  • Validated Applications: Application-tested to deliver consistent signals across common lab workflows.
  • Exceptional Lot-to-Lot Consistency: Tight lot-to-lot control for reproducible performance.
  • Customization Options: Flexible customization of tags, labels, or packaging upon request.

Custom CSF3R Membrane Protein and Antibody Discovery Services

In addition to our catalog offers, Creative Biolabs provides customized membrane protein & antibody discovery & development service. Utilizing our deep expertise and advanced platforms we can help you with:

  • Custom CSF3R Receptor Production: Tailored expression, purification, and characterization of challenging multi-domain receptor constructs.
  • Custom Antibody Development: From antigen design to antibody engineering (monoclonal, polyclonal, recombinant) for specific research applications.
  • Stable Cell Line Development: Generation of bespoke stable cell lines expressing your target of interest.
  • Functional Assay Development: Designing and executing assays to assess receptor activation and ligand.

Frequently Asked Questions (FAQ)

  1. Can the products of Creative Biolabs (CSF3R) be used for clinical purposes or in any diagnostic way?

    No, all Creative Biolabs CSF3R products and services are strictly for research purposes only and not intended for clinical diagnosis, prevention, treatment or cure of any disease These reagents are for research use only, not for use in diagnostic procedures or preclinical studies. Researchers must comply with all institutional and regulatory guidelines for the protection of human subjects.

  2. Do you furnish antibodies that discriminate between the homodimeric, G-CSF–engaged signaling-competent CSF3R and the preformed, ligand-free oligomeric pool at the myeloid progenitor surface?

    Yes, we offer confirmation-selective antibodies that react with a conformation in which the epitope is present within the membrane-proximal extracellular domain rendered accessible via receptor reorientation induced by G-CSF but sterically occluded in the basal, and preformed oligomeric structure. The reagents enable flow cytometry or immunofluorescence multiplexing to distinguish among ligand-occupied and free receptor populations on whole-cell membranes — a native measure of the dynamics of activation-state distribution without needing to maintain phospho-STAT3 after receptor engagement, nor relying on downstream readouts such as gene expression assays.

  3. Do your anti-CSF3R antibodies work for immunofluorescence staining of formalin-fixed, paraffin-embedded bone marrow trephine biopsies, splenic red pulp or G-CSF-mobilized peripheral blood smears to characterize the distribution of myeloid progenitors and mature neutrophils?

    Yes, select clones have been validated on archival hematopoietic samples after antigen retrieval and provide specific membranous staining patterns characteristic of myeloblasts, promyelocytes or mature segmented neutrophil distribution. In peripheral blood samples already mobilized by G-CSF, the intensity of staining reflects the time of exposure to G-CSF. Validation consists of peptide competition and G-CSF blocking absorption controls confirming epitope specificity.

  4. Do your CSF3R antibodies exhibit cross-reactivity with mouse and rat orthologs for translational hematopoiesis, neutropenia, and stem cell mobilization model studies?

    Yes, the immunogen is derived from a highly conserved region present in mammals that allows for species independent and ample detection of human, mouse, and rat hematopoietic preparations and corresponding bone marrow sections without needing to have multiple species specific reagents.

Reference
  1. Feng, Ziyang, et al. "CSF3R-AS promotes hepatocellular carcinoma progression and sorafenib resistance through the CSF3R/JAK2/STAT3 positive feedback loop." Cell Death & Disease 16.1 (2025): 217. Under Open Access license CC BY 4.0, without modification. https://doi.org/10.1038/s41419-025-07558-4
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