Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
Highlights
Loading...All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
Desmocollin 3 (DSC3) is a single pass transmembrane desmosomal cadherin encoded by DSC3 gene. This protein localizes to specialized plasma membrane subdomains that form desmosome intercellular junctions within epithelial cell populations. DSC3 contains multiple extracellular cadherin type repeat modules, one transmembrane helix and a sizable intracellular cytoplasmic tail. Extracellular cadherin repeats project into intercellular space and carry calcium coordination sites within each repeated unit. Cytoplasmic tail faces cell interior and bears sequence motifs dedicated to binding desmosome specific intracellular adaptor molecules. Multiple transcript isoforms exist generated by alternative splicing, differing in length and composition of cytoplasmic tail segment. Newly translated DSC3 undergoes folding and quality control processing inside endoplasmic reticulum compartments. Correctly processed protein traffics toward plasma membrane regions destined for desmosome biogenesis. DSC3 exhibits epithelial restricted expression pattern and protein abundance correlates with desmosome density in given epithelial cell layer. Only properly folded polypeptide can accumulate at nascent desmosome assembly sites. Splicing events generate distinct isoform pools which can coexist inside same epithelial cell.
DSC3 extracellular cadherin type repeats mediate heterophilic intermolecular contacts with desmoglein family cadherin molecules presented on opposing cell surfaces. Calcium ion coordination stabilizes conformation of extracellular repeat modules to sustain stable intermolecular engagement. Its cytoplasmic tail isoform variants recruit distinct sets of desmosome specific adaptor proteins to build dense multi protein plaque assemblies beneath plasma membrane. These cytoplasmic plaque complexes physically link desmosome junction sites to intermediate filament cytoskeleton networks. Other cadherin superfamily members from adherens junction systems cannot substitute for desmosome specific molecular functions performed by DSC3. Variation in DSC3 surface abundance directly influences capacity for new desmosome junction formation. Sequence disruption within extracellular cadherin repeats impairs heterophilic binding capability. Changes to cytoplasmic tail isoform composition alter spectrum of recruited plaque adaptor components and modify mechanical linkage toward cytoskeleton elements without destroying extracellular contact potential. Altered isoform balance can change the repertoire of intracellular binding partners available at desmosome sites.
Fig. 1 Schematic overview of DSC3‑containing desmosome molecular assembly. Calcium stabilizes the extracellular cadherin‑domain conformation to support intercellular adhesion. The cytoplasmic tail of DSC3 recruits desmosome‑specific adaptor components, assembling sub‑membrane plaque complexes physically coupled to intermediate‑filament cytoskeleton networks.1
The biological functions of DSC3 are focused on desmosomal heterophilic cell cell recognition, isoform dependent cytoplasmic plaque adaptor recruitment and intermediate filament cytoskeleton anchoring:
Creative Biolabs offers purified DSC3 membrane samples via standardized preparation workflows, including full length DSC3 constructs and isolated extracellular structural variants. Isolated extracellular fragments may not support complete heterophilic adhesion and isoform dependent plaque adaptor recruitment related behaviours, while full length constructs may be suited for desmosomal cadherin mediated junction assembly associated research. All samples receive routine quality screening, and functional relevant observation may only be carried out with full length samples under simulated membrane environments. All sample batches follow unified processing standards to maintain consistent structural features for comparative laboratory analysis across separate test groups.
Not finding the membrane protein product you need? Contact us to start your one-stop custom service!
Creative Biolabs provides adjustable DSC3 expression cell models with varied expression levels, applicable to transmembrane desmosomal cadherin structural characteristic observation and desmosome mediated cell cell junction related research. Sample evaluation includes sustained target expression detection and preliminary intermolecular interaction associated observation, which can support comparative analysis of desmosome associated behaviours under different expression statuses. These cell systems can be matched with diverse laboratory analysis schemes to observe changes of intermolecular contact efficiency under different target expression abundances.
Not finding the stable cell line product you need? Contact us to start your one-stop custom service!
Antibody reagents targeting DSC3 are generated via mature protein preparation workflows, compatible with multiple routine laboratory detection methods for cellular localization profiling and molecular complex identification, to support systematic analysis of DSC3 distribution and desmosome associated molecular complexes across diverse laboratory research setups. The antibody series can cooperate with other common laboratory detection reagents to complete multi dimensional observation of target distribution inside tissue samples.
Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!
Beyond catalog products, Creative Biolabs offers specialized custom services for DSC3 research:
DSC3 may function as transmembrane desmosomal cadherin and participate in calcium dependent heterophilic cell cell recognition plus isoform dependent desmosome plaque adaptor complex assembly.
DSC3 expression and isoform status may influence desmosome junction biogenesis and epithelial mechanical cell cell connectivity, serving as a major regulatory mediator of desmosome related biological processes.
No, all DSC3 related products and services are strictly for research use only, not intended for clinical related operations. All material designs and functional tests are only optimized for basic laboratory research scenarios, without matching clinical application standards.
Offerings include full length DSC3 membrane protein, target specific recombinant antibodies and adjustable expression cell research models, supporting desmosome cell cell junction molecular research.
Laboratory observation schemes may include desmosomal cadherin heterophilic interaction related tests to analyse molecule associated behaviours under simulated cellular environments.
