Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
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Dysferlin, encoded by the DYSF gene located on chromosome 2p13, is a 237 kDa membrane-associated protein belonging to the ferlin family—a group of proteins characterized by multiple C2 domains and a single C-terminal transmembrane domain. The DYSF gene is a 55-exon gene that is expressed in various tissues but predominantly in skeletal and cardiac muscles. The canonical human dysferlin protein contains 2,080 amino acids and features seven C2 domains (designated C2A through C2G) distributed along the peptide sequence, with approximately 13%–33% identity among the domains. These C2 domains function as calcium-sensitive modules that recruit calcium ions and traffic accessory proteins and vesicles to injured membrane sites. Dysferlin also contains two DysF domains and a single transmembrane domain near the C-terminus. As a single-pass type IV membrane protein, dysferlin is localized to the sarcolemma—the plasma membrane of muscle fibers—and to the transverse (T)-tubules, which are invaginations of the sarcolemma extending into the muscle fiber interior. Dysferlin binds to multiple interacting partners including caveolin-3, annexins, AHNAK, and calpain-3, consistent with its roles in membrane trafficking and repair.
Dysferlin plays a central role in maintaining the structural and functional integrity of striated muscle through multiple mechanisms. Upon mechanical stress or injury to the sarcolemma, calcium enters the cell through membrane lesions and triggers the mobilization of a dysferlin-associated repair complex that promotes membrane sealing. Dysferlin mediates the calcium-dependent fusion of intracellular vesicles with the plasma membrane to reseal membrane disruptions. In addition to its well-established role in membrane repair, dysferlin is involved in the development and maintenance of the T-tubule network. Recent evidence indicates that dysferlin regulates calcium homeostasis in striated muscle via multiple mechanisms, and this regulatory function becomes more important under conditions of stress. Mutations in the DYSF gene cause a group of autosomal recessive muscular dystrophies collectively known as dysferlinopathies, which include limb-girdle muscular dystrophy type 2B/R2 (LGMD2B/LGMDR2) and Miyoshi myopathy type 1 (MMD1). The loss of functional dysferlin leads to defective membrane repair, abnormal calcium handling, and progressive muscle degeneration. These properties make dysferlin an important research target for studies of membrane repair mechanisms, calcium signaling, T-tubule biology, and the molecular pathogenesis of muscular dystrophies.
Fig. 1 Domain structure of dysferlin.1
Dysferlin, encoded by the DYSF gene, functions as a calcium-sensitive membrane-associated protein with diverse roles in striated muscle physiology:
Creative Biolabs offers high-quality DYSF membrane protein products produced using optimized expression systems. These include full-length type IV transmembrane protein that retains the native C-terminal membrane-spanning domain, as well as isolated domain variants containing the C2 domains and DysF domains. The multiple C2 domains mediate calcium-dependent membrane binding and protein-protein interactions, making these proteins suitable for studies of membrane repair mechanisms, vesicle fusion, and protein complex assembly. All DYSF proteins undergo strict quality control to support consistent performance across applicable research platforms.
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Creative Biolabs provides custom-engineered DYSF stable cell lines, including overexpression and knockdown models. These cell lines are optimized for studies of membrane repair mechanisms, calcium signaling, T-tubule maintenance, and muscle cell biology. Each cell line undergoes stringent validation to ensure stable expression profiles and consistent functional performance in diverse experimental contexts.
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High-specificity recombinant antibodies targeting DYSF are developed using antibody engineering technologies for research applications involving DYSF expression and localization. These antibodies can be used in studies of DYSF distribution in skeletal and cardiac muscle cells and may also support characterization of DYSF-containing repair complexes in combination with appropriate detection reagents.
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Beyond catalog products, Creative Biolabs offers specialized custom services for DYSF research:
