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MADCAM1

Products

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All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

Background

Mucosal vascular addressin cell adhesion molecule 1 (MADCAM1; also known as mucosal addressin cell adhesion molecule-1) is a single-pass type I transmembrane glycoprotein of the immunoglobulin superfamily that is expressed selectively at postcapillary venules in the endothelium of mucosal and lymphoid tissues. The architecture of human MADCAM1 supports its bifunctional role in the step-wise process that leads to lymphocyte transendothelial migration. The extracellular region (residues 19–317) contains two N-terminal immunoglobulin-like domains, D1, an IgV-type domain, and D2, an IgC2-type domain, that collectively constitute the integrin-binding interface mediating α4β7-dependent adhesion. Just distal to D2 is an O-glycosylated serine/threonine-rich mucin-like domain with a negatively charged stalk that raises the Ig domains above the endothelial glycocalyx and can carry sialyl-Lewis X carbohydrates involved in L-selectin (CD62L)-mediated lymphocyte rolling. A membrane-proximal Ig-like domain present in some MADCAM1 isoforms differs between species. The protein has a single transmembrane helix (residues 318–338) embedded in the plasma membrane and a short cytoplasmic region (residues 339–382) involved in membrane-associated intracellular interactions.

Fig.1. MADCAM1 as a Drug Target. (OA Literature)Fig.1 α4β7/MAdCAM-1 as a Drug Target.1

MADCAM1 Protein Function: A Hub of Cellular Regulation

MADCAM1 has a multidimensional functional repertoire spanning mucosal immunology, lymphocyte trafficking, and the pathogenesis of inflammatory disease:

  • Lymphocyte Homing to Mucosal Tissues: MADCAM1 is expressed at a higher level on high endothelial venules and lamina propria venules in the inflamed intestinal mucosa of patients with ulcerative colitis (UC) and Crohn's disease (CD). This increased expression, promoted by proinflammatory cytokines such as TNF-α and IL-1β, assists the improper homing of α4β7+ effector T cells and memory lymphocytes into the lamina propria, leading to chronic gut inflammation through sustained damage.
  • Inflammatory Bowel Disease Pathogenesis: MADCAM1 is highly upregulated in the high endothelial venules as well as lamina propria venules of inflamed intestinal mucosa. This increased expression, which is mediated by proinflammatory cytokines such as TNF-α and IL-1β, promotes erroneous infiltration of α4β7+ effector T cells very much like-memory lymphocytes in the gut wall, thus maintaining chronic inflammation and tissue injury.
  • T Cell Costimulation and Activation: MADCAM1 acts as a strong costimulation factor for both naive and memory CD4+ T cells by recognizing integrin α4β7. Yet, in the context of T cell receptor (TCR) engagement, MADCAM1 binding alone mediates intracellular signaling pathways to yield: growth signals that increase proliferation and cytokine production. I-FN stimulates it, no doubt — but if you differentiate your CD4+ T cells in the presence of retinoic acid and TGF-β, MADCAM1 costimulation drives CCR5/CCR9 expression on CD4+T cells to generate a tissue resident memory-like cell population that is itself immunodominance-driven HIV target.

MADCAM1 Protein Product

We provide a range of high-quality MADCAM1 protein products to support structural and functional studies of this mucosal adhesion receptor. Because the extracellular region of MADCAM1 contains immunoglobulin-like and mucin-like domains with important post-translational modifications, appropriate construct design and protein format are important considerations for relevant research applications. Our products can support structural studies, α4β7-related binding research, antibody development and characterization, and other studies of MADCAM1-mediated cell adhesion. Available construct formats, tags, and other product characteristics should be determined according to the corresponding product information.

MADCAM1 Protein Product

Not finding the protein product you need? Contact us to start your one-stop custom service!

MADCAM-1 Stable Cell Line Product

Our custom-engineered MADCAM1 stable cell lines in 6 different recipient backgrounds using cell-based clones are available on request. The stable cell lines that you develop will be suitable for drug screening, lymphocyte adhesion assays under shear flow conditions, T cell costimulation studies and high-throughput screen campaigns. Our cell line development platform covers a variety of host backgrounds like HEK293, CHO-K1 or even endothelial cell lines such as HUVEC with either inducible or constitutive expression systems. We have established lines from which stable doxycycline-regulated MADCAM1 expression can be produced, enabling precise control over lymphocyte homing; derived other lines to express MADCAM1 on the apical surface of polarized epithelial monolayers for transmigration assays; and yet others where stable depletion of endogenous MAdCAM-1 enables mechanistic studies. Both flow cytometry, Western blot, and immunofluorescence localization assays are performed on each stable cell line to ensure consistent expression throughout passage as well as functional lymphocyte adhesion readouts.

MADCAM1 Stable Cell Line Product

Not finding the stable cell line product you need? Contact us to start your one-stop custom service!

MADCAM1 Recombinant Antibody Product

Our portfolio includes recombinant antibodies targeting MADCAM1, designed to support a range of research applications. Recombinant production provides sequence-defined, renewable reagents with consistent lot-to-lot production. Depending on the specific antibody and supporting datasheet, selected MADCAM1 antibodies may be suitable or available for applications such as Western Blotting, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, and other antibody-based assays. These antibodies can support the detection and analysis of MADCAM1 in appropriate research samples and model systems. Product-specific specificity, affinity, species reactivity, epitope information, conjugation options, and application compatibility should be determined according to the corresponding antibody data. For specialized research needs, customized MADCAM1 recombinant antibody discovery and development can also be discussed according to the intended experimental design.

MADCAM1 Recombinant Antibody Product

Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!

Product Features

  • High Purity & Activity: High purity and verified integrity for dependable downstream work.
  • Diverse Expression Systems: Choice of expression formats to match routine or specialized projects.
  • Species Reactivity: Products available for human, mouse, pig, and other species.
  • Validated Applications: Application-tested to deliver consistent signals across common lab workflows.
  • Superior Batch-to-Batch Consistency: Stringent quality controls to ensure low variability from batch to batch.
  • Customization Options: Tags/Labels/package Modifications available upon request.

Custom MADCAM1 Membrane Protein and Antibody Discovery Services

In addition to our catalog products, we provide dedicated custom services for membrane protein and antibody discovery & development. With a proven history and our cutting-edge platforms, we can help you with:

  • Production & Validation: Cloning of the synthesized optimized gene into an expression vector, plus small-scale expression trials to help determine the best expression conditions.
  • Custom Antibody Production: Produced via large-scale expression in mammalian/insect/cell culture or bacterial expression systems, purified in detergent or reconstituted into nanodiscs.
  • Engineered Cell Lines: Creation of stable cell lines that physiologically mimic endogenous MADCAM1 expression and activity.
  • Functional Assessment Systems: Designing and executing assays to assess protein function and ligand binding.

Frequently Asked Questions (FAQ)

  1. What MADCAM1 products do we provide?

    We provide various kinds of recombinant proteins including soluble, membrane-tethered format wild-type or domain-deleted MADCAM1 expression clones; also stable cell lines expressing proper human and mouse formatted full-length or Fc chimera with corresponding quality controls.

  2. What expression systems are recommended for MADCAM1 protein production?

    Mammalian expression system (HEK293, CHO, NS0) to achieve appropriate O-glycosylation of mucin domain for L-selectin binding and full biological activity. It is learned that Fc chimera proteins can be produced in NS0 myeloma cells and with native glycosylation. Ig domain fragments without the mucin region may also be expressed in insect cell systems.

  3. Can your anti-MADCAM1 antibodies be applied to immunofluorescence staining of formalin-fixed, paraffin-embedded intestinal lamina propria, Peyer's patch, or mesenteric lymph node sections to map high endothelial venule and follicle-associated epithelium distribution?

    Yes, selected clones were tested and are indeed positive on archival gastrointestinal specimens after antigen retrieval; they stain specifically along high endothelial venules in the lamina propria and with variable expression overlying follicle-associated epithelium of Peyer's patches. Further validation consists of peptide competition and integrin-blocking absorption controls to validate epitope specificity in addition to ligand-occupation dependence among heterogeneous tissue contexts.

  4. Do you sell stable cell lines of MADCAM1 + integrin alpha4beta7 co-expressing cells for modeling gut homing, rolling adhesion and transendothelial migration?

    Yes, we have produced dual-stable lines that constitutively express full-length MADCAM1 at one locus and integrin alpha4beta7 from another independent locus. We report a release of each batch with documentation for shear-independent binding, as well as transpiring kinetics between adhesive and stiff states within the parallel-plate flow chamber, which allows for standardized cellular platforms that exist without transfecting every time before mechanistic and therapeutic antibody screenings.

Reference
  1. Yu, Chuchu, et al. "The Role and Application of MAdCAM-1/α4β7-Induced Lymphocyte Migration in Inflammatory Enterohepatic Diseases." Biomedicines 13.11 (2025): 2659. Under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/biomedicines13112659
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