Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
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Loading...All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
The MCL1 gene is located in the 1q21.2 region of the human chromosome and encodes the myeloid cell leukemia 1 protein (MCL1), which belongs to the anti-apoptotic subfamily of the BCL-2 family. MCL1 is an integral membrane protein, with its C-terminal transmembrane region anchoring it to the mitochondrial outer membrane and its N-terminal domain exposed to the cytosol. Its molecular weight is approximately 37 kDa and it is broadly expressed across human tissues, with relatively high expression in hematopoietic tissues such as bone marrow and spleen. As a critical regulator of mitochondrial apoptosis, MCL1 binds and neutralizes pro-apoptotic BH3-only proteins such as BIM and NOXA, as well as the effector proteins BAK and BAX, thereby preventing mitochondrial outer membrane permeabilization and blocking cytochrome c release. This gene is subject to multiple regulatory mechanisms, including transcriptional control, post-translational modifications, and rapid proteasomal degradation mediated by its N-terminal PEST sequences. Phosphorylation at specific residues promotes ubiquitination and accelerates protein turnover, while caspase cleavage generates functionally distinct protein fragments. Beyond its anti-apoptotic role, a mitochondrially processed isoform of MCL1 localizes to the mitochondrial matrix, where it participates in the regulation of mitochondrial bioenergetics and cristae structure maintenance. MCL1 is frequently amplified or overexpressed in multiple cancer types, including multiple myeloma, acute myeloid leukemia, and breast cancer, where it serves as a major resistance factor against conventional chemotherapies and BCL-2-selective inhibitors such as venetoclax. Therefore, MCL1 is regarded as an important molecular target for studying apoptosis regulation, mitochondrial homeostasis, and cancer cell survival mechanisms. Its functional analysis provides valuable tools for understanding the molecular basis of apoptotic signaling and identifying approaches to overcome apoptosis-related drug resistance.
Fig. 1 Schematic of full-length USP13 (FL), full-length MCL1 (FL) and their deletion mutants (M1, M2, U1, U2).1
The functional spectrum of MCL1 is extensive and is continuously expanding:
Creative Biolabs offers a series of high-quality MCL1 membrane protein products, suitable for structural and functional research. Relying on the independently developed MemDX™ recombinant membrane protein technology platform, MCL1 proteins can be prepared through various expression systems, including virus-like particles (VLP) and in vitro prokaryotic expression systems. These rigorously validated protein products, such as MemDX™ recombinant human MCL1 membrane protein as virus-like particles and human MCL1 proteins from various expression systems, can be used in ELISA, antibody screening and identification, binding experiments, and functional research, providing reliable tools for researchers to analyze the interactions and regulatory mechanisms of MCL1.
Not finding the membrane protein product you need? Contact us to start your one-stop custom service!
Constructing a robust cell model is crucial for the study of MCL1-mediated apoptosis regulation. As an anti-apoptotic BCL-2 family member that controls mitochondrial outer membrane permeabilization, the expression level and functional status of MCL1 directly affect the threshold for apoptosis initiation and cellular sensitivity to diverse death stimuli. Different genetic backgrounds, post-translational modifications, or changes in expression levels may lead to significant differences in apoptotic priming and cell survival capacity. Creative Biolabs offers customized MCL1 membrane protein stable transfection cell models, covering wild-type and various functionally characterized forms. These models stably express the target protein on the mitochondrial outer membrane, and are suitable for studies on apoptosis signaling kinetics, protein-protein interaction analysis, BH3 profiling, and assessment of cellular dependence on MCL1 for survival, providing a reliable and reproducible platform for exploring the anti-apoptotic mechanism, structure-function relationship, and mitochondrial regulatory network of MCL1. Our technology ensures mitochondrial membrane integrity and correct protein localization, facilitating your research.
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Creative Biolabs offers highly specific recombinant antibodies targeting MCL1, produced using advanced recombinant antibody technology. Compared to traditional antibodies, these recombinant antibodies demonstrate superior performance in terms of specificity, sensitivity, and batch-to-batch consistency. They can also be modified and labeled in various ways. Our MCL1 recombinant antibodies support a wide range of applications, including Western Blot, ELISA, flow cytometry, immunofluorescence, immunocytochemistry, immunohistochemistry, and immunoprecipitation, and can be used to detect differences in MCL1 expression levels in various tissues or cell sources, analyze its subcellular localization on the mitochondrial outer membrane, and conduct related research such as protein interaction and apoptosis signaling analysis. These antibodies provide reliable detection tools for the structural analysis, functional study of MCL1, and the investigation of its regulation in the apoptotic signaling network.
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In addition to the standard products, Creative Biolabs also offers professional customized services for MCL1. MCL1 has multiple functionally distinct isoforms with significant differences in apoptotic regulatory activity. These isoforms have specific requirements in aspects such as protein expression, antigen design, and functional verification. Leveraging our technology platform, we can assist you in completing the following customized projects:
MCL1 primarily functions as an anti-apoptotic regulator on the mitochondrial outer membrane, binding to and neutralizing pro-apoptotic BH3-only proteins such as BIM and NOXA, as well as the effector proteins BAK and BAX, to prevent mitochondrial outer membrane permeabilization and maintain cell survival. The activity and stability of this protein are significantly influenced by post-translational modifications and protein turnover, and different isoforms exhibit differences in apoptotic regulatory capacity and subcellular localization. It is an important model for studying the relationship between mitochondrial membrane protein structure and function as well as the regulatory mechanism of apoptosis.
MCL1 is a critical survival factor in multiple cancer types and is frequently amplified at the 1q21.2 locus in multiple myeloma, breast cancer, and other malignancies. Its overexpression is a major mechanism of resistance to conventional chemotherapies and BCL-2-selective inhibitors. Studying the regulation and function of MCL1 is helpful for a deeper understanding of the structure-function relationship of anti-apoptotic BCL-2 proteins, the molecular mechanisms of apoptosis evasion in cancer, and the identification of strategies to overcome drug resistance.
Not applicable. All MCL1 products and services provided by Creative Biolabs are for research purposes only and cannot be used for any clinical diagnosis, prevention, treatment or cure of any disease.
We offer a variety of product types, including recombinant MCL1 membrane proteins, stable cell models expressing MCL1, high-quality recombinant MCL1 antibodies, as well as comprehensive customized services.
