Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
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Loading...All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
Mitochondrial calcium uniporter dominant negative subunit beta (MCUB) is an inner mitochondrial membrane regulatory subunit of the MCU uniporter complex, encoded by MCUB gene, widely expressed in cardiac muscle, neurons and hepatic cells, anchored to mitochondrial inner lipid bilayers. Distinct from pore-forming MCU and activating MICU subunits, MCUB acts as a constitutive inhibitory component that directly binds the MCU channel core to suppress mitochondrial Ca²⁺ influx under resting cellular states. Under physiological low cytoplasmic calcium levels, MCUB stably associates with the uniporter complex to prevent excessive calcium overload inside organelles.
MCUB executes channel inhibitory regulatory function embedded within mitochondrial inner membranes, utilizing conserved transmembrane interaction motifs to form stable complexes with the MCU pore protein. Its binding interface uniquely mediates channel suppression, separating its functional role from activating MICU family regulators. MCUB-mediated uniporter inhibition bridges cytoplasmic calcium concentration and mitochondrial energy metabolism, balancing calcium-dependent ATP synthesis and oxidative stress susceptibility according to cellular metabolic status. MCUB participates in cardiac calcium cycling, neuronal mitochondrial homeostasis and hepatic lipid-energy coupling. Deficient MCUB expression leads to uncontrolled mitochondrial calcium overload and elevated reactive oxygen species generation. Therefore, MCUB represents a pivotal research target for mitochondrial calcium channel study and anti-degenerative compound screening.
Fig. 1 Schematic of complete mitochondrial calcium uniporter complex showing MCUB as dominant-negative inhibitory subunit under resting low cytosolic calcium.1
The biological functions of MCUB are focused on uniporter complex binding and calcium uptake suppression:
Creative Biolabs offers high-quality MCUB proteins through optimized eukaryotic expression systems, including full-length inner membrane subunit and isolated MCU-binding domain variants. These products retain native channel inhibitory interaction activity, suitable for mitochondrial uniporter complex interaction and cardioprotective screening assays. All MCUB proteins undergo strict quality control to ensure consistent performance and reliable application across diverse research platforms.
Not finding the membrane protein product you need? Contact us to start your one-stop custom service!
Creative Biolabs provides custom-engineered MCUB stable cell lines, including overexpression and blank control models. These cell lines are optimized for mitochondrial channel subunit profiling and calcium flux functional analysis. Each cell line undergoes stringent validation to ensure stable expression profiles and consistent functional performance in diverse experimental contexts.
Not finding the stable cell line product you need? Contact us to start your one-stop custom service!
High-specificity recombinant antibodies targeting MCUB are developed via advanced antibody engineering technologies, with no cross-reactivity with MCU/MICU complex subunits. These antibodies are validated for inner mitochondrial membrane localization detection and cardiac/neural tissue expression profiling, and can be combined with MCU detection reagents to analyze complete uniporter complexes in primary cell models.
Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!
Beyond catalog products, Creative Biolabs offers specialized custom services for MCUB research:
MCUB is a dominant-negative inhibitory subunit of the mitochondrial calcium uniporter that binds MCU to limit excessive mitochondrial calcium influx.
MCUB sets the threshold for mitochondrial calcium uptake, critical for studying cardiac ischemia and neurodegenerative calcium toxicity mechanisms.
No, all MCUB products and services are strictly for research use only, not intended for clinical diagnosis or treatment.
Offerings include full-length MCUB membrane proteins, isoform-specific detection antibodies and custom stable cell lines for mitochondrial calcium research.
MCUB proteins are validated via MCU co-binding and mitochondrial calcium flux inhibitory functional testing.
