Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
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Loading...All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
ORAI calcium release-activated calcium modulator 1 (ORAI1) is a plasma membrane tetraspan ion channel subunit encoded by the ORAI1 gene, acting as the core pore-forming component of calcium release-activated calcium (CRAC) channels mediating store-operated calcium entry (SOCE). ORAI1 is broadly expressed across immune and epithelial cell populations, carrying evolutionarily conserved transmembrane pore helices and cytoplasmic STIM-binding interaction motifs across species, serving as an essential modulator for ER calcium store depletion-triggered calcium influx. Beyond adaptive immune cell regulation, ORAI1 also participates in the functional maintenance of innate immune subsets and epithelial barrier homeostasis, regulating cytokine secretion and cellular survival signals under persistent inflammatory stimulation. Its channel-mediated calcium flux can modulate epithelial regenerative capacity and mucosal defense response, forming a shared signaling hub connecting intracellular calcium storage balance with tissue immune surveillance. ORAI1-mediated cation permeation exerts decisive effects on sustaining sustained intracellular calcium signals required for lymphocyte activation and transcriptional programming under physiological immune conditions. Furthermore, ORAI1 coordinates downstream calcium-dependent cytokine secretion and immune cell proliferative cascades. Distinct from minor ORAI paralogs with limited tissue distribution, ORAI1 constitutes the dominant CRAC pore subunit with non-redundant immune regulatory function.
ORAI1 functions through hexameric channel assembly and physical coupling with ER-resident STIM proteins following endoplasmic reticulum calcium store depletion. The activated channel mediates sustained extracellular Ca²⁺ influx, supporting calcium-dependent signaling and intracellular calcium homeostasis. ORAI1 is involved in T-cell activation, cytokine production, and immune regulation. Loss-of-function variants can abolish CRAC currents and cause severe immunodeficiency, making ORAI1 an important research target in calcium signaling and immune disorder studies.
Fig. 1 Cryo-EM hexameric structures of wild-type Orai1 and gain-of-function V181K mutant; molecular dynamics simulations quantify pore hydration, ionic permeability and conformational shifts driven by V181X substitutions via key TM2/TM3/TM4 interfacial residues.1
The biological functions of ORAI1 are focused on tetrameric pore assembly and STIM-dependent calcium permeation for immune calcium signaling:
Creative Biolabs offers high-quality ORAI1 proteins via optimized expression systems. These products retain native tetramerization capacity and calcium permeation activity, suitable for CRAC channel electrophysiology and immune signaling compound screening. Each protein batch undergoes strict purity screening to remove incomplete fragmented protein, maintaining stable folding state during repeated experimental operations. All ORAI1 proteins undergo rigorous quality control to guarantee consistent functional performance and reliable application across diverse research platforms.
Not finding the membrane protein product you need? Contact us to start your one-stop custom service!
Creative Biolabs provides custom engineered ORAI1 stable cell lines, including overexpression and gene silencing models. These cell lines are optimized for CRAC channel electrophysiology and immune cell signaling research. Each clone is screened repeatedly to eliminate unstable subpopulations, ensuring uniform target expression level during continuous cell passaging. Each cell line undergoes strict validation procedures to ensure steady target expression levels and uniform functional performance across multiple experimental scenarios.
Not finding the stable cell line product you need? Contact us to start your one-stop custom service!
High-specificity recombinant antibodies targeting ORAI1 are developed with advanced antibody engineering workflows, without cross-reactivity against other ORAI paralogs. These antibodies receive multi-scenario functional validation, applicable to immune cell membrane localization and channel expression profiling. The preparation buffer avoids harsh preservatives, compatible with common immunofluorescence and immunoprecipitation experimental systems.
Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!
Beyond catalog products, Creative Biolabs offers specialized custom services for ORAI1 research:
ORAI1 forms the pore of CRAC channels, coupling with ER STIM proteins to mediate store-operated calcium entry and sustain immune cell calcium signaling.
ORAI1 is essential for adaptive immune activation; loss-of-function variants induce severe primary immunodeficiency, establishing it as a vital research target.
No, all ORAI1 products and services are strictly for research use only, not intended for clinical diagnosis or treatment.
Offerings include ORAI1 proteins, high-specificity recombinant antibodies and custom stable cell lines for calcium channel and immunology research.
Validation depends on the product format and may include protein purity, structural integrity, and interaction testing, while cell-based models can be used to assess STIM-dependent SOCE or CRAC current activity.
