Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
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Serine protease inhibitor A3 (also known as α1- anticchymotrypsin) is a secreted serine protease inhibitor encoded by SERPINA3 gene, which is mainly synthesized by liver parenchymal cells and activated inflammatory myeloid cells and released into the blood circulation, and can be stored in neutrophil cytoplasmic granules, which is widely involved in the steady-state regulation of systemic inflammation. Different from serine inhibitors such as A1AT, which have broad-spectrum protease inhibition, SERPINA3 has highly selective inhibitory activity and specifically targets chymotrypsin-like serine proteases such as cathepsin G and mast cell chymotrypsin. Under the physiological steady state of the body, SERPINA3 expressed in circulation can neutralize a small amount of protease leaked from granulocytes, avoid non-specific degradation of extracellular matrix and maintain the integrity of tissue structure. Under the stress of acute infection, the liver greatly upregulates SERPINA3 secretion through the acute reaction mediated by IL-6, which effectively restricts the excessive release of neutrophil protease and reduces the tissue damage caused by systemic inflammation. However, long-term chronic liver injury can continuously induce the increase of SERPINA3 expression, making it a sensitive clinical marker reflecting the progress of liver fibrosis. Other serine protease inhibitors can't compensate for the specific chymotrypsin inhibition function of SERPINA3. The deletion of SERPINA3 will significantly aggravate the organ damage mediated by inflammation. The high expression of SERPINA3 in serum can stably indicate the degree of inflammation and fibrosis, which is an irreplaceable core target for protein mechanism research and targeted screening of liver fibrosis in acute phase.
SERPINA3, as a secreted serine protease inhibitor, mainly plays a biological role in interstitial tissues and circulating body fluids, and irreversibly captures serine proteases of chymotrypsin family by means of conservative reaction center loop (RCL) structure, forming a stable inactive complex, thus terminating the protease-mediated tissue injury pathway. Its narrow and specific protease recognition spectrum forms a clear functional differentiation with broad-spectrum A1AT, and constructs the body's exclusive anti-inflammatory defense system in acute phase. SERPINA3-mediated protease neutralization effect can closely couple the acute stress signal of liver with the protective mechanism of tissue matrix, and dynamically regulate the balance between physiological inflammation clearance and pathological organ scar repair according to the duration of injury. This molecule is widely involved in many physiological and pathological processes, such as systemic acute inflammation regression, chronic liver fibrosis evolution and inflammatory degeneration of central tissue. The loss of SERPINA3 function will greatly amplify the protease activation effect stimulated by inflammation and aggravate the structural damage of tissues. To sum up, SERPINA3 is the key target for the mechanism analysis of serine protease inhibitors and the research and development of targeted therapy for liver diseases.
Fig. 1 SERPINA3 targets Glo1 to increase the expansion of NPCs, especially BPs, giving rise to an increased number of neurons and leading to cortical folding.1
The biological functions of secreted acute-phase SERPINA3 serpin are fully focused on chymotrypsin-like protease trapping and inflammatory damage restriction:
Creative Biolabs offers high-quality SERPINA3 proteins through optimized hepatic secretion systems, including full-length mature serpin and isolated reactive center loop domain variants. These products retain native chymase protease inhibitory activity, suitable for anti-inflammatory liver fibrosis screening assays. All SERPINA3 proteins undergo strict quality control to ensure consistent performance and reliable application across hepatology research platforms.
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Creative Biolabs provides custom-engineered SERPINA3 stable cell lines, including acute-phase inducible overexpression and blank empty vector control models. These cell lines are optimized for serpin protease profiling and hepatic inflammatory signal functional analysis. Each cell line undergoes stringent validation to ensure stable expression profiles during long-term hepatocyte cell culture, and can be widely deployed for anti-fibrosis compound screening experiments.
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High-specificity recombinant antibodies targeting SERPINA3 are developed via advanced antibody engineering technologies, with no cross-reactivity with other serpin A family inhibitors. These antibodies are validated for hepatocyte secretion and serum inflammatory biomarker detection and liver tissue expression profiling, and can be combined with cathepsin G detection reagents to analyze complete SERPINA3-protease inhibitory complexes in hepatic cell models.
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Beyond catalog products, Creative Biolabs offers specialized custom services for SERPINA3 research:
SERPINA3 is an acute-phase serpin that irreversibly inhibits cathepsin G and mast cell chymase to limit inflammatory tissue damage.
SERPINA3 is a sensitive circulating biomarker for acute inflammation and progressive liver fibrosis.
No, all SERPINA3 products and services are strictly for research use only, not intended for clinical liver diagnosis.
Offerings include full-length secreted SERPINA3 serpin proteins, isoform-specific detection antibodies and custom stable cell lines for hepatic inflammation research.
SERPINA3 proteins are validated via cathepsin G protease inhibitory functional testing.
