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SLC6A19

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All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

Background

Solute carrier family 6 member 19 (SLC6A19), formerly designated B0AT1 is an integral membrane protein of 540 amino acids. It is predominantly expressed in the apical membrane of epithelial cells and primarily at renal proximal convoluted tubule and small intestine but there are low levels was also detected in stomach, pancreas, liver prostate than skin. It serves as the major determinant of renal amino acid homeostasis, reabsorbing >95% neutral amino acids filtered by glomerulus in human kidney. In the intestinal tract, it mediates dietary free neutral amino acid absorption to complement peptide transport systems for efficient acquisition of nitrogen. Pathogenic variants in SLC6A19 are confirmed etiologic for the autosomal recessive disorder characterized by neutral aminoaciduria, pellagra-like skin rash and cerebellar ataxia with psychiatric phenotype. The clinical application of pharmacological inhibition of SLC6A19 is not limited to rare disease, as new research indicates potential use that involves blockade of renal reabsorption, potentially alleviating proximal tubule metabolic overload and decreasing the rate at which chronic kidney diseases progress.

Fig.1 Functional crosstalk between SLC6A19 and SLC6A14. (OA Literature)Fig.1 Functional crosstalk between SLC6A19 in the proximal small intestine and SLC6A14 in ileum and colon in the control of food intake and body weight.1

SLC6A19 Transporter Function: Orchestrating Systemic Amino Acid Homeostasis

The functional spectrum of SLC6A19 is extensive, spanning a variety of physiological and therapeutic contexts:

  • Renal Amino Acid Reabsorption: SLC6A19balances urinary nitrogen losses by retrieving filtered neutral amino acids from the glomerular filtrate, helping to establish plasma amino acid concentrations within physiological ranges. Healthy individuals excrete almost no free amino acids in urine since this reabsorptive capacity is so effective.
  • Intestinal Nutrient Absorption: Within the small intestine, SLC6A19 seizes dietary free neutral amino acids at brush border while complementing peptide transporters (PEPT1/2) to orchestrate maximal absorption of protein digestion end-products. This phenomenon is especially important for the essential amino acids that cannot be produced endogenously.
  • Metabolic Disease and Therapeutic Excretion: As in phenylketonuria (PKU), where the impairment of a phenylalanine catabolism pathway causes its toxic accumulation. By establishing a new excretory pathway that reduces plasma levels as much as 70% in preclinical models, pharmacologic inhibition of SLC6A19 also inhibits renal reabsorption of Phe. Likewise, in urea cycle disorders, improved urinary nitrogen excretion via SLC6A19 inhibition normalizes plasma ammonia and prolongs survival.

SLC6A19 Membrane Protein Product

Structural and pharmacological studies of SLC6A19 are hampered by the requirement for an appropriate lipid environment, conformational dynamics during the transport cycle, as well as the involvement of accessory proteins in surface trafficking. We tackle these challenges with a bespoke custom SLC6A19 protein design platform that provides membrane protein research constructs for structural biology, ligand-binding studies, and transport-related research. Our engineering team uses structural insights and codon-optimized gene synthesis to design constructs tailored to specific experimental requirements while considering key substrate- and ion-binding features of the transporter. For every project, you start with a comprehensive technical consultation to align the protein design architecture with your specific purpose. Specific accessory proteins, expression systems, preparation formats, and validation strategies are determined according to individual project requirements.

SLC6A19 Protein Product

Not finding the membrane protein product you need? Contact us to start your one-stop custom service!

SLC6A19 Stable Cell Line Product

Functional expression of an amino acid transport, a reliable screen for inhibitors and studies on the interaction with accessory proteins all require robust cellular models that express SLC6A19 at their apical membrane. This example demonstrates the generation of stable cell lines with homogeneous levels of a single SLC6A19 transporter by virtue of optimized transduction and selection protocols. Ours is a cell-engineering platform utilizing lentiviral delivery, transposon integration and targeted knock-in approaches for precise genetic control. Subsequently, each line is rigorously selected for clonal purity (monoclonal selection) and fully phenotyped to confirm not only surface transporter density but also sodium-dependent phenylalanine uptake activity.

SLC6A19 Stable Cell Line Product

Not finding the stable cell line product you need? Contact us to start your one-stop custom service!

SLC6A19 Recombinant Antibody Product

The availability of sequence-defined, high-affinity antibodies against SLC6A19 will be important for both detecting the transporter in kidney and intestinal tissue and mapping its apical membrane localization; indeed, similar antibodies have already been used to distinguish one member of this solute carrier family from another (SLC6A18 - B0AT3) or a different broader class altogether (e.g., Snat1/Sn at2/Slc38a4slc vs. other members that confer sodium-dependent transport). Creative Biolabs has designed comprehensive recombinant antibody discovery programs against both extracellular and intracellular SLC6A19 epitopes. Antibody Development Pipeline: We provide multi-platform selection with downstream engineering, immunogen design integrated into a single pipeline to deliver binders that possess the specificity, affinity and developability profiles for research applications. Our reproducible approach utilizes recombinant expression and clonal sequencing to remove the batch-to-batch variability that is seen with conventional polyclonal sera for a renewable, fully characterized reagent.

SLC6A19 Recombinant Antibody Product

Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!

Product Features

  • High Purity & Activity: High purity and verified integrity for dependable downstream work.
  • Diverse Expression Systems: Choice of expression formats to match routine or specialized projects.
  • Species Reactivity: Products available for human, mouse, pig, and other species.
  • Validated Applications: Application-tested to deliver consistent signals across common lab workflows.
  • Superior Batch-to-Batch Consistency: Stringent quality controls to ensure low variability from batch to batch.
  • Customization Options: Tags/Labels/package Modifications available upon request.

Custom SLC6A19 Membrane Protein and Antibody Discovery Services

Beyond catalog products, Creative Biolabs provides sophisticated discovery services covering all needs for SLC6A19 research:

  • Production & Validation: Cloning of the synthesized optimized gene into an expression vector, plus small-scale expression trials to help determine the best expression conditions.
  • Custom Antibody Production: Produced via large-scale expression in mammalian/insect/cell culture or bacterial expression systems, purified in detergent or reconstituted into nanodiscs.
  • Engineered Cell Lines: Creation of stable cell lines that physiologically mimic endogenous SLC6A19 expression and activity.
  • Functional Assessment Systems: Designing and executing assays to assess protein function and ligand binding.

Frequently Asked Questions (FAQ)

  1. Are Creative Biolabs'SLC6A19 products and services for clinical or diagnostic use?

    No. All SLC6A19 proteins, cell lines, antibodies, and custom services supplied by Creative Biolabs are designated for research use only.

  2. Do you have stable cell lines that can systematically co-express SLC6A19 with its accessory protein collectrin to continuously model renal brush-border membrane amino acid reabsorption?

    Yes, we have reliably established dual-stable lines which constitutively express full-length SLC6A19 to approximate an amount threshold for collectrin over a bolus dependence time period.

  3. Does your recombinant SLC6A19 come ready formulated for the direct reconstitution into lipid nanodiscs or planar lipid bilayers for electrophysiological and flux measurements?

    Yes, the protein is purified in a mild non-ionic detergent buffer and then subjected to size-exclusion chromatography (SEC) into a lipid-compatible system. This allows for immediate incorporation into synthetic membranes in stopped-flow fluorimetry or radiolabeled substrate accumulation assays without the need to additionally dialyze out detergent.

  4. Do your SLC6A19 antibodies cross-react with mouse and rat orthologs for translational research as they may be used in renal physiology and amino acid metabolism?

    Well, the immunogen is a region that highly conserved among all mammalian species - therefore we can get reliable detection in mouse and rat kidney and intestinal preparations without needing to use multiple various goods for each individual animal genus.

Reference
  1. Bhutia, Yangzom D., et al. "Unconventional functions of amino acid transporters: role in macropinocytosis (SLC38A5/SLC38A3) and diet-induced obesity/metabolic syndrome (SLC6A19/SLC6A14/SLC6A6)." Biomolecules 12.2 (2022): 235. Under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/biom12020235
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