Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
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The STIMATE gene is located in the 3p21.1 region of the human chromosome and encodes the STIM-activating enhancer (STIMATE), also known as transmembrane protein 110 (TMEM110), a member of the STIMATE protein family. STIMATE is a multi-pass transmembrane protein anchored in the endoplasmic reticulum membrane, with five predicted transmembrane domains and both N- and C-termini oriented toward the cytosol. Its molecular weight is approximately 33 kDa and it is broadly expressed in human tissues, with relatively higher levels detected in placenta and liver. As an important regulator of store-operated calcium entry (SOCE), STIMATE physically interacts with the ER calcium sensor STIM1 to facilitate its conformational switch and subsequent clustering at ER-plasma membrane (ER-PM) junctions, thereby promoting coupling with the ORAI1 calcium channel and activation of downstream calcium-dependent signaling pathways. These processes are involved in the regulation of calcium homeostasis, signal transduction, and the dynamic organization of membrane contact sites. The protein harbors a KxRxRR ER-retention motif essential for its subcellular localization, as well as a GXXXG motif that may contribute to oligomerization. The C-terminal region of STIMATE mediates direct binding to the CC1 domain of STIM1. Additionally, STIMATE contributes to both the long-term maintenance and short-term remodeling of ER-PM junction architecture. Therefore, STIMATE is regarded as a valuable molecular target for studying calcium signaling and inter-organelle communication. Its functional characterization provides important tools for understanding the spatial and temporal regulation of store-operated calcium entry and the molecular architecture of membrane contact sites.
Fig. 1 Main gene duplications and losses derived from the STIMATE gene family tree.1
The functional spectrum of STIMATE centers on the regulation of store-operated calcium signaling and inter-organelle communication:
Creative Biolabs offers a comprehensive series of high-quality STIMATE membrane protein products designed to support structural and functional investigations of store-operated calcium entry. Built upon the independently developed MemDX™ recombinant membrane protein technology platform, STIMATE proteins can be prepared through multiple expression systems, including virus-like particles (VLP) and in vitro prokaryotic expression systems, among others. These rigorously characterized protein products, which include MemDX™ recombinant human STIMATE membrane protein (as virus-like particles) and human STIMATE proteins derived from diverse expression systems, are suitable for ELISA, antibody screening and identification, binding experiments, and functional research, equipping investigators with dependable reagents for analyzing STIMATE-mediated regulation of STIM1 activation and calcium signaling mechanisms.
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Constructing a robust cell model is crucial for investigating STIMATE function in store-operated calcium signaling. As an ER-resident protein that promotes STIM1 conformational switching and puncta formation at ER-PM junctions, the expression level and subcellular distribution of STIMATE directly affect the efficiency of SOCE and downstream calcium-dependent signaling. Alterations in STIMATE expression or its targeting to ER-PM junctions may lead to significant changes in calcium homeostasis. Creative Biolabs offers customized STIMATE membrane protein stable transfection cell models, covering wild-type and various engineered forms. These models stably express the target protein on the endoplasmic reticulum membrane, and are suitable for studies on STIM1 interaction dynamics, calcium imaging, ER-PM junction visualization, and quantitative assessment of SOCE activity, providing a reliable and reproducible platform for exploring the molecular mechanism, structure-function relationship, and regulatory role of STIMATE in inter-organelle communication. Our technology ensures correct protein localization and ER membrane integrity, facilitating your research.
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Creative Biolabs offers highly specific recombinant antibodies targeting STIMATE, produced using advanced recombinant antibody technology. Compared to traditional antibodies, these recombinant antibodies demonstrate superior performance in terms of specificity, sensitivity, and batch-to-batch consistency. They can also be modified and labeled in various ways. Our STIMATE recombinant antibodies support a wide range of applications, including Western Blot, ELISA, flow cytometry, immunofluorescence, immunocytochemistry, immunohistochemistry, and immunoprecipitation, and can be used to detect differences in STIMATE expression levels in various tissues or cell sources, analyze its subcellular localization at ER-PM junctions, and conduct related research such as protein interaction with STIM1 and calcium signaling pathway analysis. These antibodies provide reliable detection tools for the structural analysis, functional study of STIMATE, and the investigation of its role in ER-PM communication and calcium homeostasis.
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In addition to the standard products, Creative Biolabs also offers professional customized services for STIMATE. STIMATE is a multi-pass ER-resident membrane protein containing distinct functional domains involved in STIM1 interaction and ER-PM junction targeting. These structural features present specific requirements in aspects such as membrane protein expression, antigen design, and functional characterization. Leveraging our technology platform, we can assist you in completing the following customized projects:
STIMATE functions as a positive regulator of store-operated calcium entry (SOCE) at ER-plasma membrane junctions. It physically interacts with the ER calcium sensor STIM1, facilitating its conformational switch and clustering at ER-PM contact sites to enable coupling with ORAI1 calcium channels. Through this mechanism, STIMATE modulates calcium influx and downstream calcium-dependent signaling pathways, including NFAT activation. Additionally, STIMATE contributes to the maintenance and remodeling of ER-PM junction architecture, serving as an important model for studying inter-organelle communication and calcium homeostasis.
STIMATE plays a central role in organizing ER-PM membrane contact sites, which are critical signaling hubs for calcium-dependent cellular processes. Investigating STIMATE provides insights into the molecular mechanisms governing STIM1 activation, the spatial organization of calcium signaling complexes, and the dynamic regulation of membrane contact site architecture. Understanding STIMATE function is valuable for elucidating fundamental principles of inter-organelle communication and calcium signal transduction.
Not applicable. All STIMATE products and services provided by Creative Biolabs are for research purposes only and cannot be used for any clinical diagnosis, prevention, treatment or cure of any disease.
We offer a variety of product types, including recombinant STIMATE membrane proteins, stable cell models expressing STIMATE, high-quality recombinant STIMATE antibodies, as well as comprehensive customized services.
