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TNFSF10

Products

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All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

Background

Tumor necrosis factor superfamily member 10 (TNFSF10), also known as TRAIL (TNF-related apoptosis-inducing ligand), Apo-2 ligand (Apo-2L, CD253 or TL2) has a pectic structure with a complete length of 281 amino acids and is categorized as tumor necrosis factor (TNF)20 ligand superfamily. Biological activity requires this trimeric architecture, enabling a single TNFSF10 trimer to simultaneously bind three receptor molecules, forcing the receptors into clusters that are respectively much more effective than bivalent agonistic antibodies in activating cell death. The most unique biological feature is its ability to preferentially induce apoptosis in transformed and tumorous cells but leaving most normal cells relatively unharmed; this tumor-selective cytotoxicity has gained TNFSF10 a position as one of the best new biotherapeutic agents against cancers. Such selectivity is dependent on the expression of TNFSF10 receptor types, cancer cells generally express death receptors (DR4/TRAIL-R1 and DR5/TRAIL-R2) that have functional death domains whereas normal cells upregulate decoy receptors that lack a death domain to sequester ligand without undergoing apoptosis (DcR1/TRAIL-R3 and DcR2/TRAIL-R4). TNFSF10's roles extend beyond oncology into immune surveillance, inflammation, tissue remodeling, cardiovascular protection and with emerging roles in the pathogenesis of Alzheimer's disease and the regulation of autoimmunity.

Fig.1 Hypoxia induced EMT in PTECs through miR-545-3p–TNFSF10. (OA Literature)Fig.1 Illustration of the mechanism by which hypoxia induced EMT in PTECs through miR-545-3p–TNFSF10.1

TNFSF10 Protein Function: A Precision-Guided Weapon Against Malignancy and Beyond

Its functional diversity in apoptosis induction, immune modulation, and tissue homeostasis:

  • Tumor-Selective Apoptosis via Death Receptor Clustering: TNFSF10 binding to DR4 or DR5 triggers receptor trimerization, promoting formation of the death-inducing signaling complex (DISC), followed by recruitment of Fas-associated death domain (FADD) and caspase-8 to initiate downstream apoptotic signaling.
  • Immune Surveillance and Decoy Receptor Evasion: TNFSF10 is a natural immune surveillance component, with decreased ligand expression activating immune checkpoints; NK cells and cytotoxic T lymphocytes express membrane-bound TNFSF10 to eliminate transformed cells. Tumor cells evade this host defense In part by up-regulation of decoy receptors (for exampleDcR1, andDcR2) or osteoprotegerin(OPG).
  • Non-Apoptotic Signaling and Tissue Homeostasis: TNFSF10 receptors engagement activates NF-κB, MAPK8/JNK, and PI3K/AKT pathways that promote cell survival, proliferation, and cytokines production. Stable microvessels in the cardiovascular system are regulated by TNFSF10 from endothelial cells during ischemia. At the CNS level, TNFSF10 is an important modulator of neuroinflammation, regulating microglial activation and neuronal viability in the pathogenesis of Alzheimer's disease.

TNFSF10 Membrane Protein Product

Creative Biolabs offers a comprehensive selection of high-purity recombinant TNFSF10 proteins designed to support cancer immunology and apoptosis research. Our protein expression platforms provide TNFSF10 in research-ready formats suited to different experimental requirements. These well-characterized proteins can support receptor-binding studies, apoptosis-related assays, drug screening, and mechanistic investigations of extrinsic apoptotic signaling. Specific species, oligomeric formats, tags, quality-control specifications, and available bioactivity data are provided according to the corresponding product datasheet or individual project requirements.

TNFSF10 Protein Product

Not finding the membrane protein product you need? Contact us to start your one-stop custom service!

TNFSF10 Stable Cell Line Product

The establishment of strong cellular models is essential for TNFSF10 research. Creative Biolabs is able to provide custom-engineered TNFSF10 stable cell lines expressing high levels of target TNFSF10 protein that will reliably reproduce your assay conditions. This stability makes these cell lines the best choice for drug screening, ligand binding studies, apoptosis assays, DISC formation assays, and high-throughput screening, providing a more stable and reproducible platform for studying TNFSF10 biology and pharmacology. We ensure maximum expression with cellular integrity, expediting your research pipeline. TNFSF10-overexpressing lines for studying death receptor activation and immune cell-mediated cytotoxicity were created, as were TNFSF10-knockdown lines to investigate loss-of-function effects. Following transduction, expression is validated by flow cytometry, Western blotting, and functional apoptosis assays. Developing DR5-dependent luciferase or caspase-8 activation readouts in specialized reporter cell lines allows real-time monitoring of extrinsic apoptotic signaling, making them powerful tools for research screening in cancer and autoimmune disease models.

TNFSF10 Stable Cell Line Product

Not finding the stable cell line product you need? Contact us to start your one-stop custom service!

TNFSF10 Recombinant Antibody Product

Whether you're focused on immunotherapy or other disciplines of research, we offer recombinant antibodies with high-affinity binding to TNFSF10 that can stand up to the stringent requirements today in the lab. Developed leveraging novel recombinant technologies, these antibodies offer superior specificity, sensitivity and batch-to-batch consistency over polyclonal counterparts. TNFSF10 recombinant antibodies have been tested with WB, ELISA, FC, IF and have been demonstrated to be suitable for use with serum (e.g. human), plasma (e.g. human), and various sample types including cell culture supernatants, tumor biopsies and immune cell lysates enabling the accurate detection and quantification of TNFSF10 proteins in multiple subcellular compartments used for biological study. We supply antibodies that differentiate between membrane-bound and soluble TNFSF10 isoforms, one targeting the receptor-binding domain for inhibition assays, as well as other that recognizes only the N-terminal cytoplasmic tail (for subcellular localization). Alternative agents recognizing post-translationally-modified TNFSF10 (tyrosine-phosphorylated by PKDCC/VLK) enable complex studies of ligand-activation states.

TNFSF10 Recombinant Antibody Product

Not finding the recombinant antibody product you need? Contact us to start your one-stop custom service!

Product Features

  • High Purity & Activity: High purity and verified integrity for dependable downstream work.
  • Diverse Expression Systems: Choice of expression formats to match routine or specialized projects.
  • Species Reactivity: Products available for mouse, pig, and other species.
  • Validated Applications: Application-tested to deliver consistent signals across common lab workflows.
  • Exceptional Lot-to-Lot Consistency: Tight lot-to-lot control for reproducible performance.
  • Customization Options: Flexible customization of tags, labels, or packaging upon request.

Custom TNFSF10 Membrane Protein and Antibody Discovery Services

In addition to our extensive catalog offerings, Creative Biolabs also offers customized solutions for protein engineering and antibody discovery dedicated to the unique needs of your TNFSF10 research programs:

  • Custom TNFSF10 Production: Tailored expression, purification, and characterization of challenging multi-domain receptor constructs.
  • Custom Antibody Development: From antigen design to antibody engineering (monoclonal, polyclonal, recombinant) for specific research applications.
  • Stable Cell Line Development: Generation of bespoke stable cell lines expressing your target of interest.
  • Functional Assay Development: Designing and executing assays to assess receptor activation and ligand.

Frequently Asked Questions (FAQ)

  1. Are TNFSF10 products of Creative Biolabs, your company, offered for clinical or diagnostic applications?

    No, Creative Biolabs TNFSF10 products and services are strictly intended for research use only.

  2. Do you sell antibodies that are able to distinguish between the phosphorylated, stabilized species of BNIP3 enriched at mitochondria-ER contact sites and dephosphorylated, proteasome-targeted species?

    Yes. We generated phospho-state-selective antibodies that recognize the serine-phosphorylated epitope within the N-terminal regulatory region revealed upon hypoxia-inducible factor stabilization, but masked following phosphatase-mediated deactivation.

  3. Are stable cell lines available that co-express BNIP3 with fluorescent mitochondrial?

    Yes. In such dual-stable lines, we have expressed the pro-autophagic protein BNIP3 together with either a directly mitochondrial-targeted fluorescent protein or an LC3-based autophagosome reporter from independent expression cassettes.

  4. Could your anti-BNIP3 antibodies be used on archival paraffin-embedded cardiac tissue or tumor xenograft sections or primary neuronal cultures for mapping hypoxia-gradient–dependent subcellular redistribution of BNIP3?

    Yes. Optimized clones have been confirmed on formalin-fixed specimens after a heat-mediated antigen retrieval, resulting in specific perinuclear and punctate cytoplasmic staining patterns that are enhanced towards necrotic tissue cores and co-localize with established mitochondrial markers.

Reference
  1. Kuo, Mei-Chuan, et al. "Hypoxia-Induced epithelial-to-Mesenchymal transition in proximal tubular epithelial cells through miR-545-3p–TNFSF10." Biomolecules 11.7 (2021): 1032. Under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/biom11071032
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