Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
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TRAM2, a homolog of the canonical translocon component TRAM, is an integral membrane protein of endoplasmic reticulum that shares with TRAM an N-terminal transmembrane structure that allows insertion into the Sec61 translocon channel, however the C-terminal domain diverges including a unique sequence required for protein–protein interactions. Pro-inflammatory cytokines can induce the TRAM2 gene in oral squamous cell carcinoma (OSCC).In this study, we report that TRAM2 mRNA and protein expression levels in OSCC primary tumors are significantly upregulated compared with matched normal mucosa from the same patient. Mechanistically, TRAM2 depletion restrains motility of cells such as migration, invasion and transendothelial (TEM) movement, correlated with attenuated expression of matrix metalloproteinases (MT1-MMP, MMP2, MMP9) and PERK signaling. TRAM2 promotes malignant progression of glioma via the PI3K/AKT/mTOR pathway. Moreover, a whole-genome enhancer screen revealed TRAM2 as a major modulator of YAP (Yes-associated protein) tumorigenesis, as loss of TRAM2 completely reversed YAP-induced epithelial–mesenchymal transition (EMT), YAP-dependent cellular proliferation. In hepatocellular carcinoma m6A reader RBM15B stabilizes the TRAM2 mRNA and knockdown of TRAM2 sensitizes sorafenib-resistant cells to treatment. These three converging lines of evidence have placed TRAM2 at the nexus of ER proteostasis, extracellular matrix remodeling, and oncogenic signaling.
Fig.1 Schematic representation of the calcium–collagen coupling.1
TRAM2 functional scope extends across various physiological and pathological fields with a distinct feature:
The multi-pass membrane topology of TRAM2, its association with the ER membrane environment, and its interactions with proteins such as SERCA2b complicate studies of TRAM2 structure and function. Creative Biolabs addresses these challenges with a purpose-built custom TRAM2 protein design platform that provides membrane protein constructs for structural biology and protein–protein interaction research. By leveraging structural knowledge of TRAM2 biology and codon-optimized gene synthesis, our engineering team develops constructs tailored to specific experimental requirements. All engagements begin with a technical consultation to align the desired protein architecture with downstream research applications. Specific construct formats, preparation conditions, and interaction or functional characterization strategies are determined according to individual project requirements.
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Furthermore, stable cellular systems that express functional TRAM2 at the ER membrane are essential for collagen secretion assays, calcium imaging and therapeutic antibody screens. Engineered stable TRAM2 cell lines for Creative Biolabs using transduction and selection protocols that ensure homogeneous, sustained expression. We utilize an original cell engineering platform that combines lentiviral delivery, transposon-mediated integration and targeted knock-in technologies for precise genetic control applications in vivo. After rigorous monoclonal selection for clonal purity, all lines were phenotypically characterized demonstrating both membrane localization (ER) and functional interaction with SERCA2b in addition to their procollagen synthesis capabilities.
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Our reagents are important for studying TRAM2 detection in the native tissue, determining its distribution within the ER membrane, and distinguishing it from the closely related TRAM1 paralog. Recombinant antibody discovery programs directed against the specific C-terminal domain and conformational epitopes of TRAM2 are provided form company A on an end-to-end basis. We design immunogen, select multi-platform and engineer downstream in one integrated pipeline to deliver binders with the specificity, affinity and developability profiles for research applications in antibody development. Using recombinant expression and clonal sequencing to remove the batch-to-batch variability present in conventional polyclonal sera, we provide you with an renewable fully characterized reagent since your data is limited only by your imagination.
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Creative Biolabs goes beyond traditional catalog services, providing advanced discovery solutions that address the entire TRAM2 study lifecycle:
Yes, since you are using the massive sec-independent antibody would probalby show specific for conformational epitope on TRAM2 exposed to Integration with the Sec61 translocon.
Yes, some selected clones have been validated using fixed and permeabilized cells to show reticular staining patterns with co-localization with established ER markers (e.g., calnexin). Validation by peptide competition and recombinant antigen controls to confirm epitope specificity.
Yes, we provide recombinant proteins and stable cell lines expressing published variants with changed transmembrane or loop residues. These resources allow direct comparison of complicated assembly efficiency, translocation kinetics, and membrane integration to wild-type benchmarks in controlled cellular backgrounds.
Yes the immunogen is a region that is highly conserved between mammalian species so one reagent should work in both mouse, and rat preparations without multiple species-specific reagents being required.
