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Affinity Assessment Assay

The development and evaluation of innovative drugs is an important area in which modern biological theory is transformed into actual social benefits. Creative Biolabs' drug detection platform provides customers with a comprehensive assessment of drug candidates through a series of highly efficient and reliable parameter tests, providing important parameter information for subsequent clinical trials and commercial production.

Why Affinity Assessment Assay is needed?

Molecules such as drugs and hormones that bind to receptors are considered as ligands. The binding of the ligand to the receptor is specific and reversible, and this binding can activate or inhibit the receptor and thereby affect the physiological activity of the cell. Each ligand can interact with multiple receptor subtypes. Few drugs have absolute specificity for a receptor or subtype, but most drugs are relatively selective. Selectivity is the extent to which a drug acts at a given site relative to other sites, and it is largely related to the physicochemical binding of the drug to the cellular receptor. A drug’s ability to affect a given receptor is related to the drug’s affinity, however, the affinity of the drug is not intuitive during the initial development process and requires subsequent screening and testing to select candidates for better binding to the drug receptor.

Affinity Assessment Assay

Determining the binding affinity (Kd) of the interaction between the drug and the target is not only necessary for studying the conduction and functional mechanisms of the drug but also essential for the engineering of improved drug development. Although protein interactions are theoretically easy to conceptualize, in practice, accurate measurement of affinity is a challenging task. Historically, ligand/receptor binding assays have been performed by using radiolabeled ligands, allowing for the quantification of high affinity binding interactions with high specificity. Safety concerns and limitations in radioligand labeling, along with recent advances in imaging technology have given way to fluorescent labeling and detection of ligands, which is commonly used in cell-binding assays today. A number of label-free methods have also been developed to measure protein-protein binding interactions in solution.

One common technique for measuring protein drug binding affinity is to use flow cytometry to analyze the binding of a ligand to a protein drug presented on the cell surface. However, cell-binding assays require specific considerations to accurately quantify the binding affinity of protein-protein interaction. Creative Biolabs provides an affinity method to avoid or minimize Kd by designing the basic assumptions of cell-based binding analysis, including determining the relevant equations and theories behind binding affinity. We then provide detailed protocols for direct and competitive binding assays for proteins displayed on the surface of yeast or mammalian cells, which can be used to obtain accurate Kd values.

If you have any questions about our manufacturability assessment service, you can contact us by email or send us an inquiry to find a complete solution.


All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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