Using bioreactors to produce antibody presents many advantages including better control of culture conditions, increased antibody yield, and to manufacture desired amount of antibodies. Bioreactor platforms at Creative Biolabs have proven to be a highly reliable and cost-effective alternative. Creative Biolabs provides both small and large-scale capacities in our production facility and also offers cell line optimization/adaptation services to specialize culture media for worldwide customers.
There are a variety of system types designed for harvesting monoclonal antibody (mAb) in vitro, including the static culture (in flasks), ceramic or hollow fiber matrix cartridges, and roller bottle/dialysis cartridges. Each system has distinct advantages and disadvantages. Bioreactor systems have appeared on the market designed specifically for the large-scale production of mAbs and as a non-animal use alternative to ascites mice.
Bioreactors designed for continuous culture of productive hybridoma lines are equipped with specialized cartridges to cultivate cells in dense populations and harvest mAb in concentrated amounts. Hybridoma cells are grown in a dense population without risk of poisoning themselves with the toxic metabolites that they produce. A larger volume of fresh media continually replenishes the cells with nutrients and dilutes out the waste products. It would take approximately 8-12 ascites mice to yield the same total harvest of antibody from one bioreactor cartridge. The cartridges provide an alternative to use of animals for antibody production. In some cases, hybridoma cells do not produce well in mice and a bioreactor system offers a feasible alternative for obtaining large quantities of antibody. The bioreactors also provide an antibody product that contains fewer mouse proteins and nonspecific antibodies when compare to the ascites product.
Fig.1 Factors to be considered in large-scale mAb production.
Using the bioreactor system, Creative Biolabs can produce mg to gram quantities of mAbs quickly. Our bioreactors allow the growth of antibody-producing cells at 20-30-fold higher density and therefore greatly increase secreted product concentrations, up to 100-fold. The technology adopted by the technical team allows for long-term, continuous culture from which a high concentration of antibody can be obtained for several weeks or months with standard techniques.
Creative Biolabs is a well-recognized leader in the field of antibody discovery and development. We provide antibody bulk production services to produce high specificity and sensitivity antibodies. Please contact us for more information if you are interested in our services.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.