Chemoenzymatic Synthesis of Glycoproteins

The synthesis of glycoproteins has always been a core challenge in the field of glycoprotein research. To meet the increasing demand for homogeneous glycoproteins in basic research and biomedical applications, Creative Biolabs has developed many effective glycoprotein synthesis methods. Our professional team and advanced technology can help customers solve the challenges in the chemoenzymatic synthesis of glycoproteins.

Glycosylation is a common post-translational modification of proteins. Protein glycosylation is related to many life activities. However, the research and application of glycoproteins are often limited by their structural heterogeneity. Various simple and effective methods of synthesizing glycoproteins have been utilized, which is essential for a better understanding of glycosylation in biology and the development of new diagnostic and therapeutic strategies. At the same time, enzymatic and chemical enzymatic synthesis is becoming an attractive method that complements and expands the chemical methods for constructing homogeneous glycoproteins.

Introduction of Chemoenzymatic Synthesis of Glycoproteins

The emergence of chemical enzymatic methods has promoted the synthesis of glycoproteins so that they can have a deeper understanding of their biological functions and potential therapeutic applications. The chemical enzymatic method uses endoglycosidase-catalyzed transglycosylation to link the intact oligosaccharide to the polypeptide in one step, without the participation of any protective group in this process. Enzymatic reaction intermediates as donor substrates can expand the utilization of substrates and also significantly improve the efficiency of enzymatic transglycosylation. In addition, the discovery of some new mutants with sugar synthase-like activity facilitated the synthesis of glycoproteins with full-size natural N-glycans. These advances further prove the important role of chemical enzymatic methods in glycoprotein synthesis.

Fig.1 Chemoenzymatic methods for the synthesis of glycoproteins. (Li, 2018)

Features of the Chemoenzymatic Synthesis of Glycoproteins

• Sugar chain extension without protecting groups
• Facile synthesis protocols
• Easy to expand
• Environmental friendliness
• Better atomic economy

Services in Creative Biolabs

As a leader in the field of glycoproteins, we have the key technology of glycoprotein chemical enzymatic synthesis. We have successfully applied a series of technologies used in chemical enzymatic synthesis, including natural chemical linkage, glycoprotein remodeling, and oligosaccharide chemical enzymatic synthesis. A large number of cases have been completed. We can provide a full range of glycoprotein synthesis services. Our services include:

• Glycan Extension
• Glycoprotein Remodeling for Glycoprotein Synthesis
• Enzymatic Formation of the Glycan-Protein/Peptide Link
• Glycopeptide Ligation

In recent decades, Creative Biolabs has efficiently completed a large number of glycoprotein synthesis projects. After continuous accumulation, we have established an advanced glycoprotein synthesis platform and formed an outstanding team of experts. We are pleased to provide services related to glycoprotein synthesis to global customers. Please contact us for more details.

Reference

1. Li, C.; Wang, L.X. Chemoenzymatic methods for the synthesis of glycoproteins. Chemical reviews. 2018, 118(17): 8359-8413.

Related Services:

1. Chemical Synthesis of Glycoprotein
2. Biosynthesis of Glycoprotein